摘要
[目的]研究microRNA-214-3P(miR-214)在胃癌组织中的相对表达及其与临床病理特征的关系以及对胃癌细胞GC9811、胃癌腹膜高转移潜能细胞系GC9811-P恶性表型的影响。[方法]通过qRT-PCR验证miR-214在胃癌组织及胃癌细胞系中的相对表达量,分析胃癌患者的性别、年龄、分化程度、淋巴结转移及TNM分期之间的关系;通过CCK-8方法检测细胞增殖活力,细胞划痕实验检测细胞的迁移能力,流式细胞仪实验检测细胞的凋亡能力。[结果]在25对胃癌及癌旁正常胃黏膜组织中,miR-214的相对表达量分别为2.39±0.64、0.99±0.30,P<0.05;GC9811-P细胞中miR-214的相对表达量明显高于GC9811细胞,P<0.01;miR-214的表达水平与胃癌患者淋巴结转移、TNM分期有关(P<0.05),与胃癌患者的年龄、性别及分化程度无关(P>0.05);转染慢病毒LV-miR-214 inhibitor或LV-empty lentiviral vector于GC9811-P细胞后,转染组LV-miR-214 IH的表达较对照组或空白组明显降低(P<0.01);细胞的增殖活力明显降低,细胞的迁移能力明显降低,细胞的凋亡能力明显增强;转染慢病毒LV-miR-214或LV-empty lentiviral vector于GC9811细胞后,即上调miR-214后,转染组LV-miR-214的表达较对照组或空白组明显升高(P<0.01);细胞的增殖活力明显增强,细胞的迁移能力增强,细胞的凋亡能力明显降低。[结论]miR-214在胃癌组织及胃癌腹膜高转移潜能细胞系中高表达,它的表达水平与胃癌患者淋巴结转移、TNM分期有关;对胃癌细胞的增殖活力及迁移能力具有促进作用,对细胞凋亡能力具有抑制作用。
[Objective]To analyze the relative expression of miR-214 in gastric cancer tissue and its relationship with clinicopathological characteristics, as well as its effect on malignant phenotype of gastric cancer cell line GC9811 and highly peritoneal dissemination of gastric cancer cell line GC9811-P.[Methods]Using qRT-PCR to examine miR-214 levels in human gastric cancer tissue and gastric cancer cell lines;and the relationship among gender, age, degree of differentiation, lymph node metastasis and TNM stage in gastric cancer patients was analyzed;the cell viability was assayed by CCK-8,the cell migration was assayed by scratch test and the apoptosis was tested by flow cytometry.[Results]The relative expression of miR-214 was 2.39±0.64,0.99±0.30(P<0.05)in 25 pairs of gastric cancer and adjacent normal gastric mucosal tissues, respectively, the miR-214 expression was increased in peritoneal dissemination cell line GC9811-P compared with their corresponding cell GC9811,P<0.01.The expression level of miR-214 was related to the lymph node metastasis and TNM stage in patients with gastric cancer(P<0.05),but not to the age, gender of patients and degree of differentiation with gastric cancer(P>0.05).Lentiviral miR-214 inhibitor(LV-miR-214 IH),empty lentiviral vector(LV-NC)was transfected to the GC9811-P cells, the miR-214 expression of GC9811-P cells was significantly down-regulated after Lentiviral miR-214 inhibitor transfection when compared with control or nothing, P<0.01.Lentiviral miR-214,empty lentiviral vector was transfected to the GC9811 cells, the miR-214 expression of GC9811 cells was significantly up-regulated after Lentiviral miR-214 transfection when compared with control or nothing, P<0.01.Functional assays showed that over expression of miR-214 significantly promotes cell viability, migration ability and suppress apoptosis in GC9811;down-regulation of miR-214 expression has opposite function in GC9811-P.[Conclusions]miR-214 was highly expressed in gastric cancer tissues and gastric cancer peritoneal high
作者
辛瑞娟
郭建阳
姚丽
郝倩
曹贞子
白飞虎
XIN Rui-juan;GUO Jian-yang;YAO Li;HAO Qian;CAO Zhen-zi;BAI Fei-hu(Department of Gastroenterology,Ningxia People's Hospital,750002 Yinchuan,China)
出处
《临床消化病杂志》
CAS
2022年第1期39-44,共6页
Chinese Journal of Clinical Gastroenterology
基金
国家自然科学基金资助项目(No:81760440)
宁夏自然科学基金资助项目(No:2019 AAC03161)
宁夏回族自治区人民医院培育振兴项目(No:201801)。
