摘要
目的探讨依托咪酯对胃癌细胞增殖、凋亡、迁移和侵袭的影响及可能的作用机制。方法体外培养胃癌细胞HGC-27,将细胞分为8组:正常对照组(细胞用常规培养基培养24 h)、依托咪酯组(细胞分别用含40μg·mL^(-1)依托咪酯的培养基培养24 h)、第1转染组(转染小干扰RNA阴性对照的细胞用常规培养基培养24 h)、第2转染组(转染FOXD3-AS1小干扰RNA的细胞用常规培养基培养24 h)、第3转染组(用含40μg·mL^(-1)依托咪酯的培养基培养转染空载体的细胞24 h)和第4转染组(用含40μg·mL^(-1)依托咪酯的培养基培养转染FOXD3-AS1过表达载体的细胞24 h)。用细胞计数试剂盒-8检测细胞增殖(OD值),流式细胞术检测细胞凋亡率,Transwell检测细胞迁移和侵袭,实时定量-PCR法检测细胞中叉头框转录因子D3反义RNA 1(FOXD3-AS1)基因表达(2^(-△△Ct)值)。结果与正常对照组比较,依托咪酯组HGC-27细胞增殖水平(0.32±0.02 vs 0.66±0.07,)、迁移数[(42.80±5.35)个vs(165.28±12.86)个]、侵袭数[(31.57±3.46)个vs(125.26±11.56)个]及细胞中FOXD3-AS1基因表达(0.41±0.03 vs 1.01±0.03)均显著降低,差异均有统计学意义(均P<0.05);而凋亡率升高[(29.83±2.58)%vs(2.89±0.21)%,],差异有统计学意义(P<0.05)。与第1转染组比较,第2转染组HGC-27细胞增殖水平(0.40±0.03 vs 0.67±0.06)、迁移数[(59.11±7.15)个vs(163.11±11.99)个]和侵袭数[(40.33±3.12)个vs(121.89±10.39)个]均显著降低(P<0.05),而凋亡率显著升高[(23.87±1.98)%vs(2.53±0.33)%],差异均有统计学意义(均P<0.05)。与第3转染组比较,第4转染组HGC-27细胞增殖水平(0.56±0.02 vs 0.34±0.02)、迁移数[(138.33±12.01)个vs(44.89±3.62)个]和侵袭数[(103.89±7.72)个vs 30.56±1.81)个]均显著升高,差异均有统计学意义(均P<0.05),而凋亡率显著降低[(9.96±0.73)%vs(29.39±2.36)%],差异均有统计学意义(均P<0.05)。结论依托咪酯可抑制胃癌HGC-27细胞增殖、迁移和侵袭,并诱导其凋亡,�
Objective To investigate the effect and possible mechanism of etomidate on proliferation,apoptosis,migration and invasion of gastric cancer cells.Methods Gastric cancer cells HGC-27 were cultured in vitro and divided into normal control group(cells were cultured in conventional medium for 24 h),etomidate group(cells were cultured in conventional medium containing 40μg·m L^(-1)etomidate for 24 h),transfection-1 group(cells transfected with small interfering RNA negative control were cultured in conventional medium for 24 h),transfection-2 group(cells transfected with FOXD3-AS1 small interfering RNA were cultured in conventional medium for 24 h),transfection-3 group(cells transfected with empty vector were cultured in conventional medium containing 40μg·m L^(-1)etomidate for24 h),transfection-4 group(cells transfected with FOXD3-AS1 overexpression vector were cultured in conventional medium containing 40μg·m L^(-1)etomidate for 24 h).Then cell proliferation(optical density value)was detected by cell counting kit-8.Cell apoptosis was detected by flow cytometry,and the expression(2^(-△△Ct)value)of forkhead box transcription factor D3 antisense RNA 1(FOXD3-AS1)mRNA in cells was detected by Real-time quantitative-PCR.Results The cell proliferation in the etomidate group and normal control group(0.32±0.02 vs 0.66±0.07)of HGC-27 cells,No.of migration cells(42.80±5.35 vs 165.28±12.86),No.of invasion cells(31.57±3.46 vs125.26±11.56),and the expression of FOXD3-AS1 gene in cells(0.41±0.03 vs 1.01±0.03)were decreased with significant(all P<0.05),while the apoptotic rate in the two groups[(29.83±2.58)%vs(2.89±0.21)%,P<0.05]was increased with significant(all P<0.05).The cell proliferation in transfection-2 group and transfection-1 group were(0.40±0.03 vs 0.67±0.06),No.of migration cell in the two groups were(59.11±7.15 vs163.11±11.99),and No.of invasion cell in the two groups were(40.33±3.12 vs 121.89±10.39),the apoptotic rate in the two groups were[(23.87±1.98)%vs(2.53±0.33)%];comparison between tr
作者
程浩
潘建红
吕世进
CHENG Hao;PAN Jian-hong;LU Shi-jin(Department of Anesthesiology,Affiliated Hospital of Shaoxing University of Arts and Sciences,Shaoxing 312000,Zhejiang Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2021年第21期2896-2900,共5页
The Chinese Journal of Clinical Pharmacology
基金
浙江省医药卫生科技计划基金资助项目(2020KY988)。
关键词
胃癌
依托咪酯
叉头框转录因子D3反义RNA
1
细胞增殖
迁移
侵袭
凋亡
gastric cancer
etomidate
forkhead box transcription factor D3 antisense RNA 1
cell proliferation
migration
invasion
apoptosis
