摘要
为了建立一种测定氟氯虫双酰胺有效成分的高效液相色谱分析方法,本研究采用反相高效液相色谱法,使用C_(18)色谱柱和紫外检测器,以pH=3.0甲醇-酸水(70∶30,V/V)为流动相,等度洗脱,柱温为(30.0±2)℃,流速1.0 mL/min,在230 nm波长下,对样品中氟氯虫双酰胺进行定量分析。结果表明,氟氯虫双酰胺的测定方法线性相关系数、标准偏差、变异系数分别为0.9976、0.0237%、0.47%。该方法操作简单,线性关系良好,精密度和准确度高,是测定氟氯虫双酰胺理想的分析方法。
This study aimed to establish a high performance liquid chromatography(HPLC)method for detecting fluchlordiniliprole.Determination of fluchlordiniliprole was conducted by reverse phase high performance liquid chromatography method with C_(18) column,ultraviolet detector,pH 3.0 methanol-sour water(70.0∶30.0,V/V)and isocratic elution at 230 nm detection wavelength.Column temperature and flow rate were(30.0±2)℃,1.0 mL/min,respectively.The results showed that the linear correlation coefficient,the standard deviation and coefficient of variation of fluchlordiniliprole were 0.9976,0.0237%and 0.47%,respectively.The method was an ideal analytical method of detecting fluchlordiniliprole with simple operation,good linearity,high precision and accuracy.
作者
葛家成
张永芳
闫欣欣
王丽颖
张立新
GE Jiacheng;ZHANG Yongfang;YAN Xinxin;WANG Liying;ZHANG Lixin(Hailier Pharmaceutical Group Co.,Ltd.,Shangdong Qingdao266109,China;Shenyang University of Chemical Technology,Institute of Functional Molecular,Shenyang110142,China)
出处
《现代农药》
CAS
2022年第2期40-42,共3页
MODERN AGROCHEMICALS
基金
青岛市科技计划项目(19-9-2-2-wz)。
