摘要
目的评估红景天苷对睡眠剥夺老龄小鼠围手术期神经认知障碍(perioperative neurocognitive disorders,PND)的影响。方法清洁级健康C57BL/6小鼠48只,18月龄,体重30~35 g,采用随机数字表法分为3组(每组16只):对照组(C组)、睡眠剥夺组(D组)和睡眠剥夺+红景天苷组(S组)。D组和S组采用多平台水环境法制备小鼠睡眠剥夺模型,S组于睡眠剥夺期间每日腹腔注射红景天苷50 mg/kg,连续3 d。随后3组均行脾切除手术。术后1、3 d分别取8只小鼠行Morris水迷宫实验,记录逃避潜伏期和穿越平台次数;水迷宫实验结束后处死小鼠,取海马组织,采用ELISA法测定海马组织中IL-6、IL-1β和TNF-α含量。结果与C组比较,D组和S组术后1、3 d逃避潜伏期延长(P<0.05),穿越平台次数减少(P<0.05),海马组织中IL-6、IL-1β和TNF-α含量升高(P<0.05);与D组比较,S组术后1、3 d逃避潜伏期缩短(P<0.05),穿越平台次数增加(P<0.05),海马组织中IL-6、IL-1β和TNF-α含量降低(P<0.05)。结论红景天苷可减轻睡眠剥夺老龄小鼠PND的影响,其机制可能与抑制海马炎症反应有关。
Objective To evaluate the effect of saliroside on perioperative neurocognitive disorder(PND)in sleep-deprived aged mice.Methods A total of 48 healthy clean C57BL/6 mice,aged 18 months,weighing 30‒35 g,were selected.They were divided into three groups(n=16),according to the random number table method:a control group(group C),a sleep deprivation group(group D)and a sleep deprivation and salidroside group(group S).A mouse model of sleep deprivation was established through the modified multiple platform method using mice in group D and group S.Those in group S were intraperitoneally injected with 50 mg/kg salidroside for consecutive three days during sleep deprivation.Then,splenectomy was performed in the three groups.Morris water maze test was performed on days 1 and 3 after operation.Then,the escape latency and the number of crossing the platform were recorded.Then,the mice were sacrificed and their hippocampus were collected to determine the contents of interleukin-6(IL-6),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)by enzyme-linked immunosorbent assay(ELISA).Results Compared with group C,groups D and S presented prolonged escape latency(P<0.05),a decreased number of crossing the platforms(P<0.05),and increased levels of IL-6,IL-1β and TNF-α in the hippocampus on days 1 and 3 after operation(P<0.05).Compared with group D,group S showed shortened escape latency(P<0.05),an increased number of crossing the platforms(P<0.05),and decreased levels of IL-6,IL-1β and TNF-α in the hippocampus on days 1 and 3 after operation(P<0.05).Conclusion Salidroside can relieve the effect of sleep deprivation on PND in aged mice,which may be associated with inhibiting hippocampal inflammation responses.
作者
张成成
王志萍
李静静
谈诚
张慧敏
Zhang Chengcheng;Wang Zhiping;Li Jingjing;Tan Cheng;Zhang Huimin(Department of Anesthesiology,Wuxi People's Hospital Affiliated to Nanjing Medical University,Wuxi 214023,China;Department of Anesthesiology,the Affiliated Hospital of Xuzhou Medical University,Xuzhou 221002,China)
出处
《国际麻醉学与复苏杂志》
CAS
2022年第1期13-16,共4页
International Journal of Anesthesiology and Resuscitation
基金
江苏省卫生健康委科研项目(K2019003)。
关键词
红景天苷
睡眠剥夺
围手术期神经认知障碍
小鼠
Salidroside
Sleep deprivation
Perioperative neurocognitive disorders
Mice
