摘要
为筛选鸭茅苗期抗旱性指标,以7份不同来源鸭茅(Dactylis glomerata)种质为供试材料,设置适宜水分、轻度和重度干旱胁迫3个处理,于处理30 d后测定叶片的相对含水量、相对电导率、抗氧化酶活性等生理生化指标,采用抗旱性度量值、综合抗旱系数、加权抗旱系数并结合主成分分析、隶属函数分析、灰色关联分析等多种方法对鸭茅进行抗旱性综合评价及抗旱指标的筛选。结果表明,随干旱胁迫的加剧,鸭茅叶片的相对含水量及叶绿素含量逐渐降低,相对电导率逐渐升高,渗透调节物质和丙二醛含量逐渐升高,抗氧化酶活性总体呈上升趋势。综合指标评价表明,叶片的相对含水量在轻度和重度干旱胁迫下均为敏感指标;而叶绿素含量及丙二醛含量在轻度干旱下为抗旱性敏感指标;除叶片相对含水量外,可溶性蛋白含量和可溶性糖含量在重度干旱胁迫下变化较大。综上所述,在进行抗旱性鉴定时,应用综合评价方法才能得出准确结论。对不同鸭茅种质进行比较,来自新疆的野生鸭茅在不同干旱胁迫下均表现出较强的抗旱性,具有极大的发掘潜力。
In order to screen the drought resistance indexes of Dactylis glomerata at seedling stage,7 Dactylis glomerata germplasms from different sources were used as test materials,and 3 treatments were set including suitable water,mild and severe drought stress. The relative water content,relative electrical conductivity,antioxidant enzyme activities and other physiological and biochemical indexes of the leaves were measured after 30 d of drought treatment. The drought resistance of 7 Dactylis glomerata germplasms were evaluated using drought resistance measurement value,comprehensive drought resistance coefficient and weighted drought resistance coefficient by principal component analysis,membership function analysis and gray correlation analysis. The results showed that,with the intensification of drought stress,the relative water content and chlorophyll content gradually decreased,the relative electrical conductivity, the osmotic adjustment substances and malondialdehyde content gradually increased,and the activities of antioxidant enzymes increased. Comprehensive index evaluation showed that the relative water contents of leaves were sensitive indicators under mild and severe drought stress;while the contents of chlorophyll and malondialdehyde were also sensitive indicators under mild drought,and the contents of soluble protein and soluble sugar changed greatly under severe drought stress. In conclusion,the drought resistance of Dactylis glomerata should be comprehensive evaluation. Among different Dactylis glomerata germplasms,wild Dactylis glomerata from Xinjiang showed stronger drought resistance under different drought stresses,which had great potential for exploitation for drought-resistant breeding.
作者
李江艳
张鲜花
袁小强
LI Jiangyan;ZHANG Xianhua;YUAN Xiaoqiang(Xinjiang Key Laboratory of Grassland Resources and Ecology,College of Grassland Science,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2022年第3期84-94,共11页
Journal of Agricultural Science and Technology
基金
新疆维吾尔自治区自然科学基金项目(2017D01A31)
国家自然科学基金项目(31560658)
新疆维吾尔自治区重点实验室开放课题项目(2017D04022)。
关键词
干旱胁迫
生理生化
抗旱指标
综合抗旱评价
drought stress
physiology and biochemistry
drought resistance index
comprehensive drought resistance evaluation
