摘要
目的探讨分泌性中耳炎(SOM)中耳积液中是否存在胰蛋白酶与胃蛋白酶,并比较其在儿童和成人中的浓度差别,分析其在SOM合并反流性咽喉炎(LPR)临床诊断中的价值。方法2020年1月~2021年5月,宿迁市第一人民医院收集的30例单侧SOM患者为儿童组(年龄3~9岁),徐州医科大学附属医院收集的30例单侧SOM患者为成人组(年龄26~54岁)。同期不伴中耳炎的10例正常人(年龄18~35岁)纳为对照组。所有受试者完成反流症状指数评分量表(RSI)。使用免疫印迹(WB)实验与酶联免疫吸附测定(ELISA)检测中耳积液中的胰蛋白酶与胃蛋白酶浓度。结果RSI评分>13分为阳性,儿童组阳性率为20%(6/30),成人组为33.33%(10/30),对照组均为阴性。WB结果显示,儿童组63.33%(19/30)胰蛋白酶阳性,80%(24/30)胃蛋白酶阳性;成人组60%(18/30)胰蛋白酶阳性,73.33%(22/30)胃蛋白酶阳性。儿童组与成人组中,胰蛋白酶阳性的样本中均同时检测到胃蛋白酶。ELISA结果显示,胰蛋白酶浓度:儿童组为(517.85±266.62)ng/mL,成人组为(400.50±206.15)ng/mL,2组胰蛋白酶浓度差异无统计学意义(P>0.05);胃蛋白酶浓度:儿童组为(87.12±22.38)ng/mL,成人组为62.63 ng/mL(中位数),儿童组高于成人组(P<0.05)。对照组WB和ELISA结果均为阴性。结论中耳积液胰蛋白酶与胃蛋白酶检测对于儿童SOM合并LPR有重要诊断价值。
Objective To investigate the concentration of trypsin and pepsin in the middle ear effusion of patients with secretory otitis media(SOM),to compare the concentration between children and adults,and to assess the diagnostic value of trypsin and pepsin detection in middle ear effusion for patients with SOM combined with laryngopharyngeal reflux(LPR).Methods From January 2020 to May 2021,30 children with unilateral SOM from Suqian First Hospital were collected as the children group(aged from 3 to 9 years)and 30 adults with unilateral SOM in Affiliated Hospital of Xuzhou Medical University were collected as the adults group(aged from 26 to 54 years).Ten healthy people without SOM were enrolled as the control group(aged from 18 to 35 years).All subjects completed the reflux symptom index(RSI).Western blot(WB)and enzyme-linked immunosorbent assay(ELISA)were used to detect the concentration of trypsin and pepsin in middle ear effusion.Results RSI score positive rate:20%(6/30)in children group and 33.33%(10/30)in adults group with positive RSI>13 scores.All RSI scores of the control group were negative.WB results showed that 63.33%(19/30)with trypsin positive and 80%(24/30)with pepsin positive were seen in children group.In adults group,60%(18/30)were trypsin positive and 73.33%(22/30)were pepsin positive.Pepsin was also detected in trypsin positive samples in both children group and adults group.The results of ELISA showed that the concentration of trypsin was 517.85±266.62 ng/mL in children group and 400.50±206.15 ng/mL in adults group,with no significant difference between the two groups(P>0.05).Pepsin concentration in children group was 87.12±22.38 ng/mL and in adults group was 62.63 ng/mL(median).The pepsin concentration in children group was higher than that in adults group(P<0.05).All the results of WB and ELISA in the control group were negative.Conclusions The detection of trypsin and pepsin has great value on the diagnosis of pediatric SOM combined with LPR.
作者
韩磊
赵泽祺
赵振鹿
李婷
乔月华
HAN Lei;ZHAO Zeqi;ZHAO Zhenlu;LI Ting;QIAO Yuehua(Xuzhou Medical University,Xuzhou 221000,China;Department of Otorhinolaryngology-Head and Neck Surgery,Affiliated Hospital of Xuzhou Medical University,Xuzhou 221004,China;Artificial Auditory Laboratory of Jiangsu Province,Xuzhou Medical University,Xuzhou 221000,China;School of Life Sciences,Xuzhou Medical University,Xuzhou 221000,China;Department of Otorhinolaryngology,Suqian First Hospital,Suqian 223812,China;Department of Otorhinolaryngology,Xuzhou Children’s Hospital,Xuzhou 221002,China)
出处
《中国眼耳鼻喉科杂志》
2022年第2期127-130,共4页
Chinese Journal of Ophthalmology and Otorhinolaryngology
基金
江苏省高等学校自然科学基金(18KJA320011)。
