摘要
目的建立不同辅料宁神灵颗粒多信息梯度薄层质量对比的方法。方法制备含糖型和不含糖型宁神灵颗粒供试品溶液。采用薄层色谱法,分别以环己烷-乙酸乙酯-甲酸(6∶4∶0.5,V/V/V)、三氯甲烷-乙酸乙酯-甲醇-浓氨试液(12∶1∶3∶0.5,V/V/V/V)和三氯甲烷-乙酸乙酯-甲醇-浓氨试液(1∶2∶4∶0.5,V/V/V/V)为梯度展开剂,通过紫外光灯(365,254 nm)、日光及透光检视显色前后各信息斑点的一致性。结果共检出脂溶性、中极性和水溶性成分斑点81个,归属于30余种化学成分,且不同辅料供试品溶液不同条件下的斑点显示情况均一致。结论该方法简便、快捷,可多指标、快速对比不同辅料(含糖型、不含糖型)宁神灵颗粒的质量。
Objective To establish a method for quality comparison of Ningshenling Granules with different excipients by multi-information gradient thin-layer chromatography(TLC).Methods The test solutions of Ningshenling Granules(sugar-containing type and sugar-free type)was prepared.The consistency of each information spots before and after color rendering was viewed under UV(365 nm and 254 nm),daylight and lights respectively with a mixture of cyclohexane-ethyl acetate-formic acid(6∶4∶0.5,V/V/V),trichloromethane-ethyl acetate-methanol-strong ammonia solution(12∶1∶3∶0.5,V/V/V/V)and trichloromethane-ethyl acetate-methanol-strong ammonia solution(1∶2∶4∶0.5,V/V/V/V)as gradient unfolding agents respectively by the TLC method.Results A total of 81 spots belonging to more than 30 compounds of fat-soluble,medium-polar and water-soluble components were detected,and the spots of test solutions with different excipients under different conditions were consistent.Conclusion The method is simple and rapid,which can quickly compare the quality of Ningshenling Granules with different excipients(sugar-containing type and sugar-free type)with multiple indicators.
作者
宋长国
安丽娜
蔡金凯
韩治忠
王立伟
SONG Changguo;AN Lina;CAI Jinkai;HAN Zhizhong;WANG Liwei(Chengde Yanfeng Pharmaceutical Co.,Ltd.,Chengde,Hebei,China 067600)
出处
《中国药业》
CAS
2022年第6期85-87,共3页
China Pharmaceuticals
关键词
薄层色谱法
梯度展开剂
宁神灵颗粒
含糖型
不含糖型
快速检测
TLC
gradient unfolding agent
Ningshenling Granules
sugar-containing type
sugar-free type
rapid detection
