摘要
目的观察可溶性Fms样酪氨酸激酶(soluble Fms-like tyrosine kinase,sFlt-1)、胎盘生长因子(plasma placental growth factor,PLGF)水平检测在复发性流产诊断中的意义。方法取2019年1~12月收诊的复发性流产(recurrent spontaneous abortion,RSA)患者80例设为观察组,另选同期自愿要求行人工流产的健康早孕患者80例为对照组。术前采集2组入选者肘静脉血2 ml,取血清,使用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清中PIGF及sFlt-1浓度;于人流术后留取绒毛组织,冲洗后置于4%甲醛液中固定,常规包埋、切片,使用免疫组化SP法检绒毛组织中PIGF及sFlt-1表达情况,每张切片取5个高倍镜视野,按半定量积分法行免疫组织化学评分。结果观察组血清sFlt-1浓度高于对照组,血清PIGF浓度则低于对照组,2组差异有统计学意义(P<0.05);观察组绒毛组织sFlt-1阳性表达率高于对照组,PIGF表达阳性率则低于对照组,2组差异有统计学意义(P<0.05)。并且观察组中2次流产患者血清sFlt-1浓度高于3次组及4次组,血清PIGF浓度则低于3次组及4次组(P<0.05);3次组患者血清sFlt-1浓度高于4次组,血清PIGF浓度则低于4次组(P<0.05);3组间血清sFlt-1、PIGF浓度比较,差异均有统计学意义(P<0.05)。结论复发性流产患者血清sFlt-1明显增高,PIGF浓度则明显下降,且血清sFlt-1及PIGF检测方法简便、无创、可重复性强,可作为诊断复发性流产的可靠方法。
Objective To investigate the significance of detection of soluble Fms-like tyrosine kinase(sFlt-1)and plasma placental growth factor(PIGF)levels in diagnosis of recurrent spontaneous abortion(RSA).Methods A total of 80 patients with RSA who were treated in our hospital from January 2019 to December 2019 were enrolled as observation group,at the same time,the other 80 healthy prepregnancy women who asked for induced abortion were enrolled as control group.The serum levels of PIGF and sFlt-1 before operation and after were detected by enzyme linked immunosorbent assay(ELISA)Moreover the semi-quantitative integral method was used for the immunohistochemistry grading.Results The serum levels of sFlt-1 in observation group were significantly higher than those in control group,however,the serum levels of PIGF were significantly lower than those in control group(P<0.05).The positive expression rate of sFlt-1 in villus tissues in the observation group was significantly higher than that in control group,while the positive expression rate of PIGF was significantly lower than that in control group(P<0.05).The serum sFlt-1 level in group 2 was significantly higher than that in groups 3 and 4,while the serum PIGF level was significantly lower than that in groups 3 and 4(P<0.05).The serum sFlt-1 level in group 3 was significantly higher than that in group 4,while the serum PIGF level was significantly lower than that in group 4(P<0.05).The comparison of serum sFlt-1 and PIGF levels among the three groups was statistically significant(P<0.05).Conclusion The serum levels of sFlt-1 in patients with RSA are obviously increased,while PIGF levels are significantly decreased.Moreover,the detection method of serum sFlt-1 and PIGF is simple and noninvasive,with good repeatability,therefore,it can be served as a reliable method for the diagnosis of RSA.
作者
刘静
倪素娜
邢晓琳
焦思宁
石稳
安彦敏
康峰
薄力聪
LIU Jing;NI Suna;XING Xiaolin(Shijiazhuang Hospital for Maternal and Child Health,Hebei,Shijiazhuang 050000,China)
出处
《河北医药》
CAS
2022年第6期902-904,908,共4页
Hebei Medical Journal
基金
河北省医学科学研究重点课题(编号:20201361)。
