摘要
目的建立仅与药物分子属性有关的药物分子苦度(molecule bitterness,MB)的定量方法。方法采用口尝法,以盐酸小檗碱为标准苦味物质,以不同浓度的盐酸小檗碱、奎宁、苦参碱、延胡索乙素、穿心莲内酯、栀子苷、葛根素、柚皮苷、L-精氨酸、熊果苷、益母草碱、柠檬苦素、獐牙菜苦苷、芦荟苷、龙胆苦苷、盐酸青藤碱、氧化苦参碱、京尼平苷、槐定碱的水溶液为载体,采用"最小极限法"预测苦味阈值浓度(bitterness threshold concentration,BTC),并基于此计算其MB;以盐酸小檗碱、苦参碱、氧化苦参碱、穿心莲内酯、葛根素的水溶液为载体,采用电子舌测得不同浓度溶液的响应值,基于口尝法测得的盐酸小檗碱的BTC,预测其他4种苦味单体的BTC,进而预测其MB。结果采用口尝法,测得感知到苦味的人数比例与苦味单体的浓度之间符合威布尔曲线关系,然后由拟合的曲线计算出BTC,进而测得了19种苦味单体的MB;在BTC试验的测定范围内,电子舌传感器仅对盐酸小檗碱和苦参碱的测定得到有效数据,但对其产生响应的传感器不同,不同传感器之间信息相互独立,基于电子舌未能得到苦参碱的BTC。结论采用口尝法建立了基于BTC的MB的测定方法,并测定出19种苦味单体的MB;虽未能建立基于电子舌的MB预测方法,但是为后续苦度定量方法研究提供借鉴。
Objective To establish a quantitative method of drugs’molecular bitterness(MB)which is only related to the properties of the drug molecule itself.Methods In this paper,berberine hydrochloride was used as standard bitter substance and berberine hydrochloride,quinine,matrine,tetrahydropalmatine,andrographolide,gardenoside,puerarin,naringin,L-arginine,arbutin,leonurine,limonin,swertiamarin,aloin,gentiopicroside,sinomenine hydrochloride,oxymatrine,geniposide,sophoridine with different concentrations solutions were used as research carriers,based on the minimum limit method,the bitterness threshold concentration(BTC)of bitter monomer was predicted by traditional human taste panel method,and calculated the MB using BTC finally;Using berberine hydrochloride,matrine,oxymatrine,andrographolide,puerarin solutions with different concentrations as the carriers,the response value of different concentration solutions were measured by electronic tongue.Then,the BTC of the bitter molecules were predicted,and finally the MB of the bitter monomers was predicted.Results It was measured that the relationship between the proportion of people who felt bitter and the concentration of bitterness monomer conforms to the Weibull curve by using traditional human taste panel method,then the BTC was calculated from the fitted curve,and finally the MB of 19 kinds of bitter monomers were measured;Within the measurement range of the BTC test,the electronic tongue sensors only got valid data for the measurement of berberine hydrochloride and matrine,but the sensor that responded to them were different,and the information between the different sensors were independent,so the BTC of matrine could not be obtained by using electronic tongue.Conclusion A method for the determination of MB based on BTC was established by using traditional human taste panel method,and the MB of 19 bitter monomers were determined;Although we failed to establish the MB determination method by using electronic tongue,it can be used as a reference for the further study o
作者
高晓洁
白明学
桂新景
王艳丽
王君明
姚静
张璐
施钧瀚
李学林
刘瑞新
GAO Xiao-jie;BAI Ming-xue;GUI Xin-jing;WANG Yan-li;WANG Jun-ming;YAO Jing;ZHANG Lu;SHI Jun-han;LI Xue-lin;LIU Rui-xin(Department of Pharmaceutical Preparation,Traditional Chinese Medicine Hospital of Zhengzhou,Zhengzhou 450007,China;Department of Pharmacy,the First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450000,China;Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China;Henan Modernization Engineering Research Center for Clinical Application of Traditional Chinese Medicine Decoction Pieces,Zhengzhou 450000,China;Provincial Co-construction Collaborative Innovation Center for Prevention and Treatment of Respiratory Diseases with Traditional Chinese Medicine,Henan University of Traditional Chinese Medicine,Zhengzhou 450000,China;Henan Key Laboratory for Clinical Pharmacy of Traditional Chinese Medicine,Zhengzhou 450000,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第3期696-703,共8页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金面上项目(81774452)
国家自然科学基金青年基金项目(81001646)
2017年国家重点研发计划中医药现代化重点专项(2017YFC1703402)
河南省中医药拔尖人才培养项目资助(2019ZYBJ07)
河南省高层次人才特殊支持“中原千人计划”-“中原青年拔尖人才”项目(ZYQR201912158)
河南省卫生健康中青年学科带头人专项(HNSWJW-2020014)。
关键词
最小极限法
苦味阈值浓度
分子苦度
口尝法
电子舌
盐酸小檗碱
苦参碱
minimum limit method
bitterness threshold
molecular bitterness
traditional human taste panel method
electronic tongue
berberine hydrochloride
matrine
