摘要
通过固相萃取-液相色谱-多级质谱(SPE-LC-MS/MS)联用技术和毒品胶体金免疫层析试剂盒检测法对13种中药及调味品样品中甲基苯丙胺及吗啡分别进行定量分析,依据LC-MS/MS检测结果,对毒品胶体金免疫层析试剂盒检测法进行可靠性评价。实验结果表明:型号1试剂盒对甲基苯丙胺和吗啡的特异性均不高,检测准确率分别为57.7%与78.8%;型号2试剂盒对甲基苯丙胺的特异性不足,准确率为73.1%,但对吗啡的检测准确率达到100%。在利用毒品胶体金免疫层析试剂盒进行毒品快速筛查时,应注重排除干扰因素以提高免疫胶体金层析试剂盒的检测准确度。
Methamphetamine and morphine existing in 13 kinds of traditional Chinese medicine and condiment samples were quantitatively analyzed by SPE-LC-MS/MS and diagnostic kits(colloidal gold).According to the results of LC-MS/MS,the reliability of diagnostic kits(colloidal gold)was evaluated.The results showed that Type 1 diagnostic kit had no high specificity to both methamphetamine and morphine,with detection accuracy of 57.7%and 78.8%,respectively.Type 2 diagnostic kit had low specificity to methamphetamine with detection accuracy of 73.1%but exhibited high specificity to morphine with the detection accuracy of 100%.Thus,in order to improve the detection accuracy,interference should be emphatically eliminated when the diagnostic kit(colloidal gold)is used for rapid drug detection.
作者
陈晓洁
范鑫雨
张大海
李先国
CHEN Xiaojie;FAN Xinyu;ZHANG Dahai;LI Xianguo(College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100)
出处
《分析试验室》
EI
CAS
CSCD
北大核心
2022年第2期201-206,共6页
Chinese Journal of Analysis Laboratory
基金
中国海洋大学基本科研业务费项目(201762030)资助。
关键词
精神活性物质
固相萃取-液相色谱-多级质谱
胶体金免疫层析法
psychoactive substances
solid phase extraction-liquid chromatography-multi-stage mass spectrometry
immune colloidal gold chromatography
