摘要
目的：研制吗啡-甲基安非他明单克隆一步法毒品二合一胶体金检测试卡，用于毒品的检测和可疑人群的筛查。方法：应用杂交瘤技术制备抗吗啡-甲基安非他明的单克隆抗体（McAb）；ELISA方法检测McAb效价；免疫胶体金技术制备二合一胶体金检测试卡。结果：@ELISA法检测McAb的效价：抗吗啡McAb的效价为1：3．2×10^3，抗甲基安非他明McAb的效价为1：1．6×10^3。②检测试卡的灵敏度：检测尿中吗啡的最低浓度为300ng／ml，甲基安非他明的最低浓度为1000ng／ml；③检测试卡的特异性：7天以上未吸毒者饮用可口可乐、口服感冒药及吸烟等其尿中未检出阳性反应。④与Pan Probe Bio-tech公司的单一检测吗啡和甲基安非他明试纸灵敏度对比实验显示出吸毒后4～5天的尿样仍呈阳性反应。结论：由于具有高度敏感性和特异性，以及快速、直观等优势，本试卡在毒品滥用筛查工作中有重要的定性判别意义。
Objective ：To prepare a colloid gold kit for the qualitative detection of the dopes met-amphetamine and morphine by one-step chromatography immunoassay in human samples based on the principle of the highly specific immunochemical reactions between the antigens and their antibodies which were used for the analysis of specific compounds in human urine samples at ideal cut-off concentrations. Methods：The monoclonal antibodies（McAb） of either mouse anti-morphine or mouse met-amphetamine were prepared by hybridomas and the limiting dilution respectively. The titers of McAb were tested by ELISA. The kit was assembled and the specificity, sensitivity, and stability were investigated. In addition, these characters were compared with those of issued Pan Probe single test strip. Results：（1）The titer of the first McAb against morphine was 1： 3. 2×10^3 , as for another, 1：1.6×10^3. （2）The colloid gold kit was found with following cut-off for the dope concentrations, morphine at 300 ng/ml and met-amphetamine at 1 000 ng/ml. （3）The colloid gold kit showed fine specificity without cross-reaction with routine medicines for cold treatment or with the solf-drinks of cokocola or with tobacco for the individuals who did not take the dopes the 7 days. （4）The colloid gold kit was even more sensitive than the issued strip,because urine sample of the junkies were still positive 4-5 days post-intake. Conclusion：The colloid gold kit for indentifying the drug-users is a rapid, specific, sensitive immunoassay suitable for the simultaneous, qualitative detection of drug abuse.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2007年第7期637-640,共4页
Chinese Journal of Immunology
关键词
单克隆抗体
胶体金
灵敏度
特异性
Monoclonal antibody（McAb）
Colloid gold
Sensitivity
Specificity
