摘要
目的探讨当归芍药散对非酒精性脂肪性肝病(NAFLD)大鼠肠黏膜机械屏障的保护机制。方法60只雄性SD大鼠随机分为正常组、模型组、易善复组和当归芍药散低、中、高剂量组,每组10只。通过喂饲高脂饲料制备NAFLD大鼠模型,造模同时予相应药物干预,连续8周。半自动生化分析仪检测大鼠血清总胆固醇(TC)、三酰甘油(TG)、丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)及肝组织TC、TG含量;手动生化法检测肝组织游离脂肪酸(FFA)含量;ELISA检测血清脂多糖(LPS)含量;HE染色观察肝脏、回肠和结肠病理形态,测量小肠绒毛高度和宽度;油红O染色观察肝脏脂质蓄积;免疫组化检测回肠和结肠组织闭合蛋白(occludin)、闭锁小带蛋白-1(ZO-1)表达。结果与正常组比较,模型组大鼠血清TC、TG、ALT、AST、LPS和肝组织TC、TG、FFA含量明显增加(P<0.01),肝脏脂肪变性明显,回肠和结肠黏膜上皮细胞出现水肿,小肠绒毛高度、宽度及回肠、结肠组织occludin、ZO-1蛋白表达明显降低(P<0.01);与模型组比较,当归芍药散各剂量组大鼠血清TC、TG、ALT、AST、LPS和肝组织TC、TG、FFA含量明显减少(P<0.05,P<0.01),肝脏脂肪变性和肠道病理损伤均减轻,小肠绒毛高度、宽度及回肠、结肠组织occludin、ZO-1蛋白表达明显升高(P<0.05,P<0.01)。结论当归芍药散对NAFLD大鼠具有较好的治疗作用,其机制可能与保护肠黏膜机械屏障、降低肠道通透性有关。
Objective To investigate the protective mechanism of Danggui Shaoyao Powder on the mechanical barrier of intestinal mucosa of rats with nonalcoholic fatty liver disease(NAFLD).Methods Totally 60 male SD rats were randomly divided into normal group,model group,Yishanfu group and Danggui Shaoyao Powder low-,medium-and high-dosage groups,with 10 rats in each group.High fat diet were fed to establish the NAFLD rat model,and each treatment group was given corresponding drugs at the same time for 8 consecutive weeks.Semi-automatic biochemical analyzer was used to detect the contents of total cholesterol(TC),triglyceride(TG),glutamic pyruvic transaminase(ALT),glutamic oxaloacetic transaminase(AST)in serum,and the contents of TC and TG in liver tissue;manual biochemical method was used to measure the content of free fatty acid(FFA);ELISA was used to detect the content of lipopolysaccharide(LPS)in serum;HE staining was used to observe the pathological morphological changes of liver,ileum and colon,and the height and width of small intestinal villi;oil red O staining was used to observe the lipid accumulation of liver;immunohistochemistry was used to detect the proteins expression of occludin and ZO-1 in ileum and colon tissues.Results Compared with the normal group,the contents of TC,TG,ALT,AST and LPS in serum,the contents of TC,TG and FFA in liver tissue of the model group significantly increased(P<0.01),the hepatic steatosis was obvious,the edema of epithelium cells of ileum and colon was seen,the height and width of small intestinal villi and the proteins expression of occludin and ZO-1 in ileum and colon significantly decreased(P<0.01).Compared with the model group,the contents of TC,TG,ALT AST and LPS in serum,and the contents of TC,TG and FFA in liver tissue of Danggui Shaoyao Powder low-,medium-and high-dosage groups significantly decreased(P<0.05,P<0.01),the hepatic steatosis and intestinal pathological damage significantly alleviated,the height and width of small intestinal villi and the proteins expression of o
作者
彪雅宁
储心乔
刘晨旭
韩雪
张焱东
韩一鹭
张一昕
BIAO Yaning;CHU Xinqiao;LIU Chenxu;HAN Xue;ZHANG Yandong;HAN Yilu;ZHANG Yixin(School of Pharmacy,Hebei University of Chinese Medicine,Application Technology Research Center of Traditional Chinese Medicine Prescription in University of Hebei Province,Shijiazhuang 050200,China;Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2022年第3期65-71,共7页
Chinese Journal of Information on Traditional Chinese Medicine
基金
河北省自然科学基金面上项目(H2020423028)
河北省高等学校科学研究计划重点项目(ZD2021079)
河北省中医药管理局科研计划项目(2020127)
国家级大学生创新创业训练计划项目(202114432006)。
关键词
非酒精性脂肪性肝病
当归芍药散
肠黏膜机械屏障
肠道通透性
大鼠
nonalcoholic fatty liver disease
Danggui Shaoyao Powder
intestinal mucosal mechanical barrier
intestinal permeability
rats
