摘要
采集了77份云南省疑似感染莴苣褪绿病毒(lettuce chlorosis virus,LCV)的番茄样本,采用特异性反转录PCR扩增LCV次要外壳蛋白基因(minor coatprotein,CPm)近全长序列,常规Sanger测序法测定相应片段序列,并对其进行遗传进化分析。结果表明,LCV特异性反转录PCR扩增得到与预期大小(1.38 kb)—致的特异性条带;采集的77份番茄样本中LCV检出率为12.99%。测定的LCV CPm;发育分析表明,所有LCV分离物可分为3个亚簇,具有明显的地域特性,其中LCV云南分离物与中国其他地区LCV分离物聚为一亚簇,来自美洲和欧洲分离物分别聚为一个簇。遗传进化分析表明,LCV云南分离物YN58有1个重组事件,序列中嘧啶(C/T)替换率最高(25.25),呈现独立进化、加速扩展趋势。这是在云南省首次报道发现LCV。
The 77 tomato leaves suspected to be infected by lettuce chlorosis virus(LCV)were sampled in Yunnan Province,China. The reverse transcriptional(RT)-PCR amplified near full length of minor coat protein gene(CPm)of LCV,which was sequenced by Sanger sequencing method. The genetic evolution was analyzed based on the sequences of near full length CPm genes. The results showed that the specific 1.38 kb fragment was amplified by RT-PCR,and 12.99% LCV-positive samples were detected in the 77 tomato leaf samples. The sequences of near full length CPm genes of Yunnan Province,China,shared the highest nucleotide sequence identity of 99.27% – 99.78% with LCV Shandong isolate(MK370741). Phylogenetic analysis indicated that all of LCV isolates were divided into three subclusters with region-dependent,LCV Yunnan isolates grouped into same subcluster with other LCV isolates of China,and America isolates and European isolates grouped into other two subclusters separately. Genetic evolution analysis revealed one recombinant event exiting in LCV Yunnan isolate YN58,and the highest nucleotide substitution of 25.25 occurring in pyrimidine C/T,which shown LCV had the tendency of independent evolution and expedited diffusion in China. This is the first report of LCV in Yunnan Province.
作者
冯丽肖
胡荣
卜姗
张德咏
罗香文
李凡
丁铭
张卓
张松柏
刘勇
FENG Lixiao;HU Rong;BU Shan;ZHANG Deyong;LUO Xiangwen;LI Fan;DING Ming;ZHANG Zhuo;ZHANG Songbai;LIU Yong(College of Longping,Graduate School of Hunan University,Changsha 410125,China;Plant Protection Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China;Plant Protection College Yunnan Agricultural University,Kunming 650201,China;Yunnan Academy of Agricultural Sciences,Kunming 650231,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第1期141-147,共7页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31972242)
国家现代农业产业技术体系专项资金项目(CARS-23-D-02)。
关键词
番茄
莴苣褪绿病毒
分子检测
系统发育分析
进化
tomaoto
lettuce chlorosis virus
molecular detection
phylogenetic analysis
evolution
