摘要
根据已报道的烟草线条病毒外壳蛋白基因序列,设计出一对特异性引物,提取大豆病叶的总RNA反转录成cDNA,PCR扩增出约400bp大小的产物,产物克隆到pGEM-TEasy载体上,转化大肠杆菌DH5α,抽提的质粒用NotI酶切,出现430bp大小的目标插入片段。经序列测定确认了插入片段的大小为404bp。
A pair of specific primers was designed based on the conserved region of the coat protein gene of Tobacco streak virus. Total RNA was extracted from infected soybean leaf and was reverse transcribed to cDNA. The cDNA was subjected to PCR using the forward and reverse primers. PCR product was cloned into pGEM T-Easy vector and transformed the E. coli DH-5α. Target fragment about 430bp was found when the inserted plasmid was digested with Not Ⅰ. The inserted fragment was sequenced. It consisted of 404 nucleotides.
出处
《植物检疫》
2006年第3期136-138,共3页
Plant Quarantine
基金
国家质量监督检验检疫总局的经费支持(2002IK015-01)
