摘要
旨在探究新型鹅细小病毒(novel goose parvovirus,NGPV)与宿主细胞相互作用的分子致病机制。本研究采用转录组测序技术对感染NGPV SD株14 d雏鸭的肝、胸腺和回肠进行分析。结果显示:感染组回肠上调基因有78个,下调基因有31个;感染组胸腺上调基因有111个,下调基因有78个;感染组肝中上调基因有128个,下调基因有313个;NGPV感染雏鸭后,胸腺、回肠和肝中的差异表达基因参与免疫效应过程、淋巴细胞介导的免疫等多个免疫相关生物学过程,并在Toll样受体信号通路、JAK-STAT信号通路、NF-κB信号通路、PI3K-Akt信号等多条信号通路富集。综上表明:NGPV感染激活了机体免疫反应和宿主细胞的相关抗病毒信号通路。此研究为NGPV的致病机制研究提供了新的线索。
This experiment was conducted to explore the molecular pathogenic mechanism of the interaction between novel goose parvovirus(NGPV)and host cells.In this study,transcriptome sequencing was performed on the liver,thymus and ileum of ducklings 14 days after infection with NGPV SD strain.The results showed that there were 78 up-regulated genes and 31 down-regulated genes in the ileum of the infected group;111 up-regulated genes and 78 down-regulated genes in the thymus of the infected group;128 up-regulated genes and 313 down-regulated genes in the liver of the infected group.The differentially expressed genes in thymus,ileum and liver of ducklings infected with NGPV are involved in many immune related biological processes,such as immune response process and lymphocyte mediated immunity.The differential genes are enriched in multiple signaling pathways such as Toll-like receptor signaling pathway,JAK-STAT signaling pathway,NF-κB signaling pathway,PI3K-Akt signaling.It is suggested that NGPV infection activates the body’s immune response and the relevant antiviral signal pathways of host cells.This study provides new clues for the study of the pathogenic mechanism of NGPV.
作者
王璐瑶
郝雪飘
雷白时
赵款
张武超
袁万哲
WANG Luyao;HAO Xuepiao;LEI Baishi;ZHAO Kuan;ZHANG Wuchao;YUAN Wanzhe(College of Animal Medicine, Hebei Agricultural University, Baoding 071001, China;Hebei Veterinary Biotechnology Innovation Center, Hebei Agricultural University, Baoding 071001, China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2022年第2期654-657,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
河北省重点研发计划(18227517D)
河北农业大学引进人才科研专项(YJ202020)。
关键词
新型鹅细小病毒
短喙侏儒综合征
转录组测序
免疫反应
novel goose parvovirus
short beak and dwarfism syndrome
transcriptome sequencing
immune response
