摘要
为研究生长因子受体结合蛋白2(Growth factor receptor-bound protein 2,GRB2)和SOS(Son of sevenless)基因组在籽鹅等级前卵泡中的表达情况,本研究采用高通量测序技术对籽鹅等级前卵泡进行转录组测序分析,之后通过荧光定量PCR进行验证。通过FPKM值进行分析,GRB2和SOS在籽鹅等级前卵泡中均有表达。随着卵泡的发育,GRB2在小白卵泡中表达量最低,在初级卵泡中表达量最高;SOS在大白卵泡和中白卵泡中的表达量最低,在初级卵泡中的表达量最高。荧光定量PCR结果与转录组测序结果基本一致。
The expression of growth factor receptor-bound protein 2 （GRB2） and Son of sevenless （SOS） in the pre-follicles of Zi goose was studied by transcriptome sequencing technology. The Zi-goose pre-hierarchical follicles transcriptome was sequencing with the Illumina Hiseq 2500 and the result was indicated by fluorescence quantitative PCR. The results showed that GRB2 and SOS gene all were expressed in pre-hierarchieal follicles though computational analysis the FPKM value. With the development of follicles,the expression of GRB2 had the minimum expression level in the middle white follicles, and the primary follicles had the highest expression. The expression of SOS had the minimum expression level in the large white follicles and the middle white follicels, and the primary follicles had the highest expresssion. The transcriptome results were basically consistent with fluorescent quantitative PCR.
出处
《中国家禽》
北大核心
2017年第9期5-10,共6页
China Poultry
基金
吉林农业大学首批青年拔尖人才支持计划(2016)
吉林省留学回国人员择优资助项目(2015)
吉林省科技厅重点科技攻关(20150201025NY)
国家科技部星火计划(2015GA660001)
吉林省牧业局(2016)
关键词
籽鹅
等级前卵泡
生长因子受体结合蛋白2
转录组测序
Zi goose
pre-hierarchical follicles
growth factor receptor-bound protein 2
transcriptome
