摘要
【目的】利用高通量测序技术获得大青的转录组信息特征。【方法】通过高通量测序平台Illumina HiSeq;2500对大青进行转录组测序,采用Trinity软件de novo组装获得Unigene,并基于序列同源性对Unigene进行功能注释,得到大青转录组的遗传信息。【结果】测序数据经过质控后共获得26 394 223个高质量的Reads,通过de novo组装获得100 191个Unigene,N50长度为1 055 bp,平均长度724.4 bp。其中59 690个(59.58%)Unigene在NR、SwissProt、KOG、GO、KEGG数据库中均得到注释。其中KEGG数据库注释到38 260个Unigene,涉及136条代谢通路。在大青转录组中共鉴定到407个Unigene参与萜类化合物的生物合成,165个Unigene涉及类黄酮生物合成,29个Unigene参与黄酮和黄酮醇生物合成,37个Unigene参与异黄酮生物合成,同时,还鉴定到210类转录因子。MISA分析发现6 680个Unigene包含8 640个简单重复序列。【结论】利用高通量测序技术和生物信息分析获得了大青的转录组信息特征,这些数据可为后期开展功能基因鉴定、解析黄酮类化合物次生代谢途径及其调控机制奠定研究基础。
Objective To obtain the transcriptome information characteristics of Clerodendron cyrtophyllum Turez by high-throughput sequencing technology. Methods The transcriptome sequencing of Clerodendron cyrtophyllum Turez was performed by high-throughput sequencing platform Illumina HiSeq;2500. Unigenes were de novo assembled by Trinity software,and then the function annotation of unigenes was carried out based on sequence homology to obtain the genetic information of Clerodendron cyrtophyllum Turez transcriptome. Results After quality control of sequencing data,a total of 26 394 223 high quality reads were obtained,100 191 unigenes were obtained through de novo assembly,and the N50 length was 1 055 bp,with an average length of 724.4 bp. A total of 59 690(59.58%)unigenes were annotated in NR,SwissProt,KOG,GO and KEGG databases. Among them,38 260 unigenes were annotated in the KEGG database,involving 136 metabolic pathways. A total of 407 unigenes were identified in the transcriptome of Clerodendron cyrtophyllum Turez involved in terpenoid biosynthesis, 165 unigenes involved in flavonoid biosynthesis, 29 unigenes involved in flavone and flavonol biosynthesis, 37 unigenes involved in isoflavonoid biosynthesis, and 210 transcription factors(TFs)were also identified. MISA analysis showed that 6 680 unigenes contained 8 640 simple sequence repeats(SSRs). Conclusion High-throughput sequencing technology and bioinformatic analysis are used to obtain the transcriptome information characteristics of Clerodendron cyrtophyllum Turez, and these data will lay a foundation for later research on functional gene identification and analysis of secondary metabolic pathways of flavonoids and their regulatory mechanisms.
作者
刘敏
黄炜忠
何孟璐
梅瑜
王继华
LIU Min;HUANG Wei-Zhong;HE Meng-Lu;MEI Yu;WANG Ji-Hua(Key Laboratory of Crops Genetics and Improvement of Guangdong Province/Crop Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640 Guangdong,China;Guangdong Luofushan Sinopharm Co.,Ltd.,Huizhou 516100 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2022年第1期177-183,共7页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
以农产品为单元的广东省现代农业产业技术体系创新团队建设项目(南药产业)(编号:2019KJ148)
科技创新战略专项资金(高水平农科院建设)-金颖之星(编号:R2019PY-JX003)
广东省博罗县南药产业园项目(2019)。
关键词
大青
萜类
黄酮
转录组
简单重复序列(SSR)
Clerodendron cyrtophyllum Turez
terpenoid
flavonoid
transcriptome
simple sequence repeats(SSR)
