摘要
目的探讨电针"百会""人中"穴治疗脑缺血再灌注损伤(CIRI)的疗效及可能作用机制。方法36只雄性SD大鼠随机分为假手术组、模型组和电针组,每组12只。除假手术组外其余大鼠采用大脑中动脉线栓阻断法制备CIRI大鼠模型。造模24 h后对电针组大鼠行电针干预,穴取"百会""人中",频率15 Hz、强度为1 mA的连续波,每天1次,每次20 min,共5天。用Longa法评定各组大鼠神经功能损伤程度;TTC染色法测定大鼠脑梗死体积,HE染色观察脑组织病理形态改变;ELISA法检测脑脊液白细胞介素1β(IL-1β)、白细胞介素6 (IL-6)、白细胞介素18 (IL-18)水平;逆转录聚合酶链反应和Western blotting法分别检测皮质缺血区脑组织Gasdermin D (GSDMD) mRNA和Gasdermin D蛋白的氮端(GSDMD-N)蛋白表达。结果与假手术组比较,模型组大鼠神经功能评分明显上升、脑梗死体积明显增加,脑脊液IL-1β、IL-6和IL-18含量明显增加,皮质缺血区脑组织GSDMD mRNA及GSDMD-N蛋白表达水平明显增高(P<0.05或P<0.01);HE染色结果显示,模型组大鼠脑组织神经元排列紊乱,部分神经细胞消失,胞核固缩,结构不完整。与模型组比较,电针组大鼠神经功能评分下降、脑梗死体积减小,脑脊液IL-6和IL-18含量明显减少,GSDMD mRNA及GSDMD-N蛋白表达水平降低(P<0.05或P<0.01);大鼠大脑皮质神经元变性及神经细胞数量丢失程度均较模型组有所减轻。结论电针"百会""人中"穴能使CIRI模型大鼠脑梗死体积减小,改善大鼠神经功能,其机制可能与下调脑脊液炎症因子和皮质缺血区脑组织细胞焦亡底物蛋白GSDMD表达有关。
Objective To explore the effect of electroacupuncture(EA) at Baihui(DU 20) and Renzhong(DU26) on cerebral ischemia reperfusion injury(CIRI) and the possible mechanism.Methods A total of 36 male SD rats were randomized into sham-operation group(n=12),model group(n=12) and EA group(n=12).Suture middle cerebral artery occlusion was used to develop the CIRI model in all groups but the sham-operation group.Twenty-four hours after modeling,the rats in the EA group were performed for EA at Renzhong and Baihui,acupoints with a continuous wave with intensity of 1 mA and frequency of 15 Hz,once daily,20 min each time,for 5 days.The Longa method was used to evaluate the degree of neurological function injury in the rats;TTC staining was used to measure the cerebral infarct volume of rats;HE staining was used to observe the pathological and morphological changes in brain tissue;ELISA method was used to detect the levels of interleukin 1β(IL-1β),interleukin 6(IL-6) and interleukin 18(IL-18) in cerebrospinal fluid;reverse transcription polymerase chain reaction and Western blotting were performed to determine the expressions of Gasdermin D(GSDMD) mRNA and the N-terminal fragment of GSDMD(GSDMD-N) protein in brain tissue.Results Compared to the sham-operation group,the model group had higher neurological function score and cerebral infarct volume,increased levels of IL-1β,IL-6 and IL-18 in the cerebrospinal fluid,as well as increased expressions of GSDMD mRNA and GSDMD-N protein(P<0.05 or P<0.01).HE staining showed that the neuron arrangement in the brain tissue of the model group was disordered;some of the nerve cells disappeared;and the nucleus was constricted with incomplete structure.Compared to the model group,the EA group had significantly decreased neurological function score,cerebral infarct volume,levels of IL-6 and IL-18 in the cerebrospinal fluid,and expression levels of GSDMD mRNA and GSDMD-N protein(P<0.05 or P<0.01);and the neurons degeneration in the cerebral cortex and the loss of the number of nerve cells we
作者
罗敷
封蔚彬
刘丹妮
孙光华
周桂娟
谭金曲
黄夏荣
尹林伟
刘红雅
周君
LUO Fu;FENG Weibin;LIU Danni;SUN Guanghua;ZHOU Guijuan;TAN Jinqu;HUANG Xiarong;YIN Linwei;LIU Hongya;ZHOU Jun(The First Affiliated Hospital of Hengyang Medical College,University of South China,Hengyang,421001;West China Hospital,Sichuan University)
出处
《中医杂志》
CSCD
北大核心
2022年第2期164-168,共5页
Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81973917)
湖南省自然科学基金(2020JJ5508)
湖南省卫健委2020年度科研立项课题(20200474)。
关键词
脑缺血再灌注损伤
电针
百会
人中
炎症因子
细胞焦亡底物蛋白
cerebral ischemia reperfusion injury
electroacupuncture
Baihui(DU 20)
Renzhong(DU 26)
inflammatory factors
Gasdermin D
