摘要
目的本研究旨在观察三七总皂苷(Panax notoginseng saponins,PNS)对A549细胞上皮-间充质转化(Epithelial-mesenchymal transition,EMT)的影响及其机制。方法将A549细胞随机分为5组:正常对照组、TGF-β1诱导组(5 ng·mL^(-1),诱导24h)、PNS干预组(低、中、高浓度组分别加入TGF-β15 ng·mL^(-1)和50、100、200μg·mL^(-1)PNS);倒置显微镜下观察各组细胞的形态变化;Elisa法检测细胞上清液中Ⅰ型胶原蛋白(COLⅠ)的表达;Western blot检测各组E-cadherin、Vimentin、磷酸化p38MAPK(P-p38MAPK)蛋白的表达。结果倒置显微镜下TGF-β1诱导的A549细胞由圆形变为长梭形、纺锤形,细胞圆形度降低(P<0.01),PNS干预后细胞圆形度增高(P<0.05);Elisa法检测显示,与正常对照组相比TGF-β1诱导组细胞上清液COLⅠ表达增高(P<0.05),与TGF-β1诱导组比较PNS干预后COLⅠ表达降低(P<0.05);Western blot检测与与TGF-β1诱导组比较,PNS干预后E-cadherin蛋白表达增加(P<0.05),Vimentin蛋白表达降低(P<0.05),磷酸化的p38丝裂原活化蛋白激酶(P-p38 MAPK)表达降低(P<0.05)。结论三七总皂苷可能通过调控TGF-β1/p38MAPK信号通路可以抑制TGF-β1诱导A549细胞发生EMT现象。
Objective To investigate the effect of Panax Notoginseng Saponins(PNS)on epithelial-Mesenchymal transition(EMT)of A549 cells and its mechanism.Methods A549 cells were randomly divided into 5 groups:normal control group,TGF-β1 induction group(5 ng·mL^(-1),24 h induction),and PNS intervention group(low,medium and high concentration groups were added with TGF-β15 ng·mL^(-1)and 50,100 and 200μg·mL^(-1)PNS,respectively).Morphological changes of each group were observed under inverted microscope.Elisa method detected the cell that the supernatant fluid typeⅠcollagen(COLⅠ)expression;The expression of E-cadherin,Vimentin and phosphorylated p38MAPK(P-p38MAPK)were detected by Western blot.Results Under inverted microscope,TGF-β1 induced A549 cells changed from round to long spindle and spindle shape,and the cell roundness decreased(P<0.01).After PNS intervention,the cell roundness increased(P<0.05).Elisa method detection showed that compared with the normal control group,the expression of COLⅠin the cell supernatant of the TGF-β1 induction group increased(P<0.05).Compared with the TGF-β1 induced group after PNS intervention,COLⅠexpressed lower(P<0.05);Compared with TGF-β1 induced group,the expression of E-cadherin protein was increased after PNS intervention(P<0.05),the expression of Vimentin protein was decreased(P<0.05),and the expression of phosphorylated P38 mitogen-activated protein kinase(P-p38 MAPK)was decreased(P<0.05).Conclusion Total saponins of Panax notoginseng may inhibit TGF-β1induced EMT in A549 cells by regulating the TGF-β1/p38MAPK signaling pathway.
作者
王莹莹
丁玉文
闫五玲
李瑞琴
王白燕
刘湘花
张彩丽
孙春霞
曾小虎
Wang Yingying;Ding Yuwen;Yan Wuling;Li Ruiqin;Wang Baiyan;Liu Xianghua;Zhang Caili;Sun Chunxia;Zeng Xiaohu(School of Basic Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Medical College,Zhengzhou 450046,China;The Third Affiliated Hospital,Henan University of Chinese Medicine,Zhengzhou 450046,China;Academy of Chinese Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2021年第10期3772-3778,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
河南省中医药管理局河南省中医药研究专项课题普通课题(2017ZY2109):栝楼薤白汤含药血清抗博来霉素诱导的大鼠肺泡巨噬细胞致纤维化作用及机制研究,负责人:孙春霞、李瑞琴
河南省教育厅河南省高等学校重点科研项目(17A310020):瓜蒌薤白汤通过JNK/P38MAPK途径对人肺泡上皮细胞上皮间质转化的干预作用,负责人:李瑞琴。
关键词
上皮-间充质转化
P38MAPK
肺纤维化
三七总皂苷
Epithelial-mesenchymal transformation(EMT)
p38MAPK
Pulmonary fibrosis
Panax notoginseng total saponins
