摘要
本文建立HPLC波长切换法同时测定蒙药钦汤中没食子酸、原儿茶酸、羟基红花黄色素A、栀子苷和鞣花酸的含量的方法.试验结果表明,测定的没食子酸、原儿茶酸、羟基红花黄色素A、栀子苷、鞣花酸分别在检测范围内(0.358-0.954μg、0.066-0.175μg、0.386-1.031μg、1.685-4.493μg、0.308-0.822μg)保持良好的线性关系;平均加样回收率为98.8%-101.8%,RSD<2.0%;3批样品中5个成分的含量分别是1.0399-1.0629、0.0778-0.0863、1.0986-1.1844、6.5152-6.836 3、1.2444-1.3098 mg·g^(-1).该方法简便、准确,可同时测定钦汤中5种活性成分,适用于钦汤质量的控制.
An analytical method for determining gallic acid, protocatechuic acid, hydroxysafflor yellow A, gardenoside and ellagic acid in Qintang of Mongolian medicine was established by high performance liquid chromatography( HPLC) wavelength switching. The results showed that the five components of gallic acid, protocatechuic acid, hydroxyl safflower yellow A, gardenoside and ellagic acid were maintain a good linear relationship within the detection range(0.358-0.954 μg、0.066-0.175 μg、0.386-1.031 μg、1.685-4.493 μg、0.308-0.822 μg). The average recoveries were 98.8%-101.8%, RSD < 2.0%. The contents of 5 components in 3 batches of samples were1.0399-1.0629,0.0778-0.0863,1.0986-1.1844,6.5152-6.8363,1.2444-1.3098 mg·g^(-1),respectively. The method is simple, accurate, and can simultaneously determine five active ingredients in Qintang, and is suitable for the quality control of Qintang.
作者
杨雪苗
康松松
冯智翱
邬国栋
安明
薄彧坤
YANG Xuemiao;KANG Songsong;FENG Zhiao;WU Guodong;AN Ming;BO Yukun(Baotou Medical College,Baotou,014040,China)
出处
《环境化学》
CAS
CSCD
北大核心
2021年第12期3986-3988,共3页
Environmental Chemistry
基金
内蒙古自治区科技创新引导项目内蒙古自治区财政厅(00120209)
包头医学院大学生创新创业训练计划(29)
内蒙古自治区高等学校科学研究项目(NJZY19193)
内蒙古自然科学基金(2021MS08011)资助。
