摘要
目的:建立蛇床子中蛇床子素的含量测定方法。方法:采用高效液相色谱法,色谱柱选择Agilent ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-体积分数0.05%磷酸溶液(体积比70:30),流速为1.0 mL/min,检测波长为322 nm,柱温30℃。结果:蛇床子素的线性关系方程为Y=4.172×10^(4)X+1.912×10^(2),在12.5~200μg/mL范围内的峰面积与进样浓度线性关系良好(R^(2)=0.9996)。精密度、重复性及稳定性试验的相对标准偏差(RSD)分别为1.28%、1.32%、0.87%,平均加样回收率为99.36%,RSD为1.53%。蛇床子中蛇床子素的平均含量为5.58 mg/g。结论:建立的高效液相色谱法方法简便、准确、快速,重复性好,专属性好,可应用于蛇床子中蛇床子素的含量测定。
Objective:To establish a method for the determination of osthol in fructus cnidii.Methods:The HPLC was performed on Agilent ZORBAX SB-C18 column(250 mm×4.6 mm,5μm)with mobile phase consisted of methanol-0.05%phosphoric acid solution(70:30 by volume)at a flow rate of 1.0 mL/min.The detection wavelength was set at 322 nm.The column temperature was 30℃.Results:The linear relationship equation of osthol was y=4.172×10^(4)X+1.912×10^(2),the peak area in the range of 12.5-200μg/mL had a good linear relationship with the injection concentration(R^(2)=0.9996).The RSDS of precision,repeatability and stability tests were 1.28%,1.32%and 0.87%respectively.The average recovery was 99.36%,and the RSD was 1.53%.The average content of osthol in fructus cnidii was 5.58 mg/g.Conclusion:The established HPLC method is simple,accurate,rapid,reproducible and specific.It can be applied to the determination of osthol in fructus cnidii.
作者
林进生
Lin Jinsheng(Fujian Pientzehuang Cosmetics Co.,Ltd.,Fujian 363000,China)
出处
《中国野生植物资源》
CSCD
2022年第1期44-46,共3页
Chinese Wild Plant Resources
