摘要
为建立饲料中替米考星含量的高效液相色谱检测分析方法,实验样品经水-乙腈-磷酸二丁胺溶液-四氢呋喃(805:115:25:55,V:V:V:V)提取后,振荡10 min,超声10 min,4000 r/min离心5 min,取上清液过0.45μm有机微孔滤膜后供高效液相色谱仪分析测定。结果表明:替米考星在10~500μg/mL质量浓度范围内线性关系良好,相关系数大于0.999,方法检出限为5 mg/kg,定量限为10 mg/kg,平均回收率为92.0%~101.2%,相对标准偏差为1.02%~5.17%。该方法操作简单,重复性好,准确率高,且上机8 min即可完成替米考星的检测,适用于饲料中替米考星含量的测定。
To establish a method of high performance liquid chromatography(HPLC)for determination of tilmicosin in feed.The sample was extracted by water-acetonitrile-dibutyl phosphate solution-tetrahydrofuran(805:115:25:55,V:V:V:V),vibrated for 10 min,ultrasonically extracted for 10 min and centrifugated at 4000 r/min for 5 min.The supernatant was filtered by 0.45μm organic microporous membrane and analyzed by HPLC.The results showed that the linearity of tilmicosin was good with the correlation better than 0.999 within the range of 10~500μg/mL.The limits of detection was 5 mg/kg,and the limits of quantification was 10 mg/kg.The average recovery of the method was 92.0%~101.2%with the relative standard deviation(RSD)of 1.02%~5.17%.The method was simple,repearable,accurate and could be achieved in less than 8 min,which was suitable for the detection of tilmicosin in feed.
作者
牛蓉
秦丽波
张梦丽
周兰兰
NIU Rong;QIN Libo;ZHANG MengLi;ZHOU Lanlan(Agricultural Food Testing Center,Henan Hongxin Testing Technology Co.,Ltd.,Nanyang,Henan Province 473000,China;The Quality Control Department,Muyuan Foods Co.,Ltd.,Nanyang,Henan Province 473000,China)
出处
《中国饲料》
北大核心
2022年第3期103-107,共5页
China Feed
关键词
饲料
替米考星
高效液相色谱法
feed
tilmicosin
high performance liquid chromatography
