摘要
目的:探索甘姜苓术汤(GJLZ)的活性成分,并探讨其治疗类风湿关节炎(RA)的机制。方法:采用超高液相色谱-串联四级杆飞行时间质谱仪联用技术(UPLC-Q-TOF/MS)检测GJLZ中药物成分,并采用网络药理学分析GJLZ治疗RA的作用靶点及通路,并采用分子对接和细胞实验进行验证。结果:采用UPLC-Q-TOF/MS筛选出GJLZ药物成分15个,网络药理学得到对应靶点177个,RA疾病靶点2 048个,药物和疾病交集靶点19个。京都基因与基因组百科全书(KEGG)及基因本体注释(GO)分析得到20个生物学功能和通路,分子对接结果表明GJLZ中3个成分(姜烯酮-A、姜烯酮-B、甘草酚)与核心靶点对接能力较好。体外细胞实验显示GJLZ可显著降低IL-17、VEGF表达,抑制炎症因子释放,防止RA发生。结论:甘姜苓术汤通过靶向抑制IL-17、VEGF表达发挥RA治疗作用。
Objective:To explore active ingredients of Ganjianglingzhu Decoction(GJLZ)and its mechanism of anti-rheumatoid arthritis(RA).Methods:Ultra-high liquid phase-tandem quadrupole time-of-flight mass spectrometer technology(UPLC-Q-TOF/MS)to detect effective ingredients of GJLZ,and network pharmacology to analyze targets and pathways of GJLZ in RA treatment,and verified by molecular docking and cell experiments.Results:15 GJLZ drug ingredients were screened out by UPLC-Q-TOF/MS.Network pharmacology analysis revealed 177 corresponding targets,2048 RA disease targets,and 19 drug and disease intersection targets.Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)analysis obtain 20 biological functions and pathways.Molecular docking results showed that three components of GJLZ(Gingerenone-A,Gingerenone-B,Glycyrol)had good docking ability with core target.In vitro cell experiment showed that GJLZ significantly reduced expressions of IL-17 and VEGF,inhibited release of inflammatory factors,and prevented occurrence of RA.Conclusion:GJLZ plays a role in treatment of RA by targeting expressions of IL-17 and VEGF.
作者
陈少波
位佳琳
何蕊
邢春来
初洪波
位鸿(指导)
CHEN Shaobo;WEI Jialin;HE Rui;XING Chunlai;CHU Hongbo;WEI Hong(Changchun University of Chinese Medicine,Changchun 130117,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2022年第3期301-307,I0001-I0008,共15页
Chinese Journal of Immunology
基金
吉林省科技发展计划项目(20190304077YY)。
