摘要
背景:3D生物打印的应用愈加广泛,与之相关的生物墨水灭菌则非常重要,然而用于临床目的的生物墨水的灭菌问题尚未得到解决。目的:对用于3D生物打印的生物墨水灭菌技术的研究做一综述。方法:检索中国知网、万方数据、PubMed和Web of Science数据库中相关文献,中文检索词为“3D生物打印、组织工程、增材制造、生物墨水、生物材料、灭菌、杀菌、无菌化”,英文检索词为“3D bioprinting,tissue engineering,additive manufacturing,bio-ink,biomaterials,sterilization,disinfect,aseptic”。最终纳入77篇相关文献进行综述。结果与结论:不同的灭菌方法在完成对3D生物打印的生物墨水灭菌的同时也会对生物墨水产生不良影响。①有些灭菌方法可损伤生物墨水的微观结构:比如射线辐射灭菌与紫外线产生的自由基会影响生物墨水的流体力学和可塑造性,过氧乙酸因其强氧化性溶解生物墨水的某些成分,对机械性能要求较高的材料应减少使用此种灭菌方法;②有些灭菌方法可破坏生物墨水所含生物大分子的活性:比如高压蒸汽灭菌的高能量水蒸汽、射线辐射灭菌与紫外线产生的自由基会导致蛋白质和酶等生物大分子的活性丧失,因此对需要生物大分子发挥活性的生物墨水应减少使用此种灭菌方法;③有些灭菌方法可残留毒性物质:如环氧乙烷灭菌后的残留物有致癌性,影响种子细胞的生存,所以对需要负载种子的生物墨水应减少使用此种灭菌方法;④有些灭菌方法对生物墨水不能完成彻底灭菌:如乙醇、抗菌素等方法在灭菌时受其自身性质的限定不能总是完成对生物墨水的灭菌,因此常需要与其他方法的联用,例如不同种类抗菌素的联用、过氧乙酸与乙醇联用等既能实现优势互补还不造成额外不良影响;⑤因此选择最适用于3D生物打印的生物墨水灭菌方法时应综合考虑材料本身特性、材料应
BACKGROUND:The application of 3D bioprinting is becoming more and more extensive,and the related bio-ink sterilization is very important.However,the sterilization problem of bio-ink used for clinical purposes has not been resolved.OBJECTIVE:To summarize the current research progress of bio-ink sterilization technology for 3D bioprinting.METHODS:Retrieve relevant documents were retrieved on CNKI,Wanfang Data Platform,PubMed,and Web of Science databases,with the search terms of“3D bioprinting,tissue engineering,additive manufacturing,bio-ink,biomaterials,sterilization,disinfect,aseptic”.Finally,77 relevant articles were included for review.RESULTS AND CONCLUSION:Different sterilization methods have bad effects on bio-ink for 3D bioprinting while sterilizing bio-ink.(1)Some sterilization methods can damage the microstructure in the biological ink.For example,radiation sterilization and ultraviolet light produced free radicals can affect biological fluid mechanics and plasticity of ink.Acetic acid peroxide dissolved biological ink ingredients due to its strong oxidizing.The mechanical performance requirements of high material should reduce to use this kind of sterilization method.(2)Some sterilization methods can damage the activity of the biological ink containing biological macromolecules,such as autoclaving of high-energy water vapor,radiation sterilization,and ultraviolet light produced free radicals can lead to loss of biological macromolecules such as protein and enzyme activity.Therefore,biological ink that requires biological macromolecules to be active should reduce to use this kind of sterilization method.(3)Some sterilization methods will remain some toxic substances:for example,ethylene oxide sterilization residues have carcinogenicity and affect the survival of seed cells,so the biological ink that needs to be loaded with seeds should reduce the use of this sterilization method.(4)Some sterilization methods for ink cannot complete a thorough sterilization.For example,ethanol and antibiotic during ste
作者
苑龙
李森
卞继超
李万祥
王国栋
Yuan Long;Li Sen;Bian Jichao;Li Wanxiang;Wang Guodong(Department of Clinic,Jining Medical University,Jining 272000,Shandong Province,China;Affiliated Hospital of Jining Medical University,Jining 272000,Shandong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第18期2914-2921,共8页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81871814),项目负责人:王国栋
济宁市重点研发计划(2020YXNS022),项目负责人:王国栋。
关键词
3D生物打印
组织工程
增材制造
生物墨水
生物材料
灭菌
杀菌
无菌化
3D bioprinting
tissue engineering
additive manufacturing
bio-ink
biomaterials
sterilization
disinfect
aseptic
