摘要
比较3个白木香良种‘热科1号’(Aquilaria sinensis ‘Reke 1’)、‘热科2号’(A. sinensis ‘Reke 2’)、‘热科3号’(A. sinensis ‘Reke 3’)及国外引种的印度沉香(A. agallocha Roxb.)植物叶片挥发油成分的异同,旨在为其进一步开发利用提供依据。采用水蒸气蒸馏法提取4种叶片中的挥发油,用气相色谱-质谱联用(GC-MS)技术对其成分进行分析获得总离子流图,通过Date Analysis化学工作站,结合Nist2005和Wiley275质谱库及前人鉴定方法等对检测到的色谱峰进行检索及人工解析;利用峰面积归一化法测定其化学成分的相对百分含量,统计数据后进行对比分析。结果表明,3个白木香良种与印度沉香叶片挥发油仅5个共有成分,独有成分数量较多占半数以上;‘热科1号’(34.44%)、‘热科3号’(25.08%)及印度沉香(37.06%)叶片挥发油中萜类总相对百分含量较高,‘热科2号’(13.20%)中相对较低,其中‘热科1号’(9.82%)与‘热科2号’(8.17%)沉香叶挥发油中分别有3种、8种倍半萜类化合物且均为独有;‘热科2号’(5.03%)沉香叶挥发油中二萜类成分远低于其他三者(‘热科1号’为19.25%、‘热科3号’为24.18%、印度沉香为35.45%);‘热科1号’(5.37%)与印度沉香(1.61%)叶片挥发油中含三萜类成分,其他二者未检出。综上,萜类多具有抗菌抗炎活性,故‘热科1号’‘热科3号’及印度沉香叶片挥发油更适合发掘其在药用、保健方面的功能,印度沉香叶挥发油的二萜类化合物中致香成分含量较高,适合发掘香料、烟草等方面的应用价值,‘热科2号’叶挥发油具有苦味的二萜类化合物含量低,在食品、茶饮方面具有较好的开发潜能及应用前景。因此,对不同沉香树叶及挥发油的开发利用应更具针对性,充分挖掘其价值,开发高附加值产品并推动沉香树种植产业调整升级,促进沉香产业发展。
To analyze the similarities and differences of the volatile oil constituents from the leaves of three improved varieties of Aquilaria sinensis(Lour.) Spreng, A. sinensis ‘Reke 1’, A. sinensis ‘Reke 2’, A. sinensis ‘Reke 3’ and a introduced foreign species A. agallocha Roxb., which could provide a basis for further development and utilization. The volatile oil from the leaves were extracted by steam distillation, and its components were analyzed by gas chromatography-mass spectrometry(GC-MS) to obtain total ion flow diagrams. Through the Date Analysis Chemistry workstation and combined with NIST2005 and WILEY275 mass spectrometry libraries and previous identification methods, the detected chromatographic peaks were retrieved and analyzed manually. The peak area normalization method was used to determine the relative content of each chemical constituent, and the statistical data were compared and analyzed. There were only 5 common components in the volatile oil from the leaves of the three improved varieties of A. sinensis and A. agallocha, and the number of unique ingredients accounted for more than half. The content of total relative percentage of terpenes in the volatile oil of the leaves of ‘Reke 1’(34.44%), ‘Reke 3’(25.08%) and A. agallocha(37.06%) were higher, while that of ‘Reke 2’(13.20%) was relatively lower. There were 3 and 8 unique sesquiterpenoids in the volatile oil of ‘Reke 1’and ‘Reke 2’, respectively. The diterpenoids in the volatile oil from the leaves of ‘Reke 2’(5.03%) were much lower than those of the other three [‘Reke 1’(19.25%), ‘Reke 3’(24.18%) and A. agallocha(35.45%)]. The volatile oil of the leaves of ‘Reke 1’(5.37%)and A. agallocha(1.61%) contained triterpenoids which were not detected in the other two. In conclusion, terpenoids mostly have antibacterial and anti-inflammatory activities, so the volatile oil of the leaves of ‘Reke 1’ ‘Reke 3’ and A.agallocha are more suitable for exploring the functions of medicinal and health-care.
作者
刘欣怡
王雅丽
王昊
王露露
梅文莉
戴好富
王军
LIU Xinyi;WANG Yali;WANG Hao;WANG Lulu;MEI Wenli;DAI Haofu;WANG Jun(College of Forestry,Hainan University,Haikou,Hainan 570228,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Hainan Agarwood Engineering Technology Research Center/Hainan Key Laboratory of Research and Development of Natural Product from Li Folk Medicine,Haikou,Hainan 571101,China)
出处
《热带作物学报》
CSCD
北大核心
2022年第1期196-206,共11页
Chinese Journal of Tropical Crops
基金
2019年海南省重点研发计划科技合作方向项目(No.ZDYF2019203)
2020年中央财政林业技术科技推广示范资金项目(琼[2020]TG05)。
关键词
白木香良种
印度沉香
叶片挥发油
GC-MS分析
improved varieties of Aquilaria sinensis
A.agallocha
leaves volatile oil
GC-MS analysis
