摘要
分别由酸疮痂链霉菌(Streptomyces acidiscabies,SA)、茄科雷尔氏菌(Ralstonia solanacearum,RS)和胡萝卜软腐欧文氏菌(Erwinia carotovora subsp.carotovora Borgey,ECCB)引起的植物疮痂病、青枯病、软腐病是农作物生产中常见多发的细菌病害。番石榴叶挥发油由多种活性物质组成,具有广谱抗微生物活性。本研究旨在提取并配置番石榴叶挥发油抗菌液,针对酸疮痂链霉菌、茄科雷尔氏菌、胡萝卜软腐欧文氏菌等3种病原菌进行挥发油的抗性研究,为农作物病害生物防治储备资源。采用添加锂盐结合微波辅助水蒸馏法提取番石榴叶挥发油,通过滤纸片扩散法和最小抑制浓度(MIC)评价了番石榴叶挥发油对3种供试菌株的抗菌效果,单因素结合响应面试验优化提取工艺,采用气相色谱-质谱联用技术(GC-MS)测定番石榴叶挥发油的化学成分。结果表明:番石榴叶挥发油对酸疮痂链霉菌、茄科雷尔氏菌、胡萝卜软腐欧文氏菌均表现出良好的抑制效果,抑菌圈直径范围为20.57~23.24 mm,抑菌率均达到60%以上,MIC值分别为3.13、1.56、3.13μg/mL。挥发油对茄科雷尔氏菌的抑制效果最好,抑菌圈直径为(23.24±0.47)mm,属于高度敏感,对酸疮痂链霉菌、胡萝卜软腐欧文氏菌也具有一定的抑制效果。提取的最佳工艺条件为:液料比10 mL/g(干重)、微波功率660 W、微波时间43 min,在此条件获得的挥发油得率为(10.23±0.17)mL/kg(干重),3次平行试验验证了该方法的有效性和重现性。检测出番石榴叶挥发油的主要成分为萜烯类、醇类等物质,其中β-石竹烯(28.36%)、去氢白菖烯(11.47%)、蓝桉醇(11.31%)、α-蒎烯(10.01%)的含量相对较高;抗菌试验中,发挥主要作用的物质为β-石竹烯、α-蒎烯、丁香酚、D-柠檬烯,推测番石榴叶挥发油对供试菌株的抑菌机理是对其细胞结构造成了破坏,导致细胞内物质泄漏。上述结果表明,番石榴叶挥发油具有良好�
Streptomyces acidiscabies(SA),Ralstonia solanacearum(RS)and Erwinia carotovora subsp carotovora borgey(ECCB)cause plant scab,brucellosis and soft rot of carrot,respectively.These are common and prevalent bacterial disease in crop production.The volatile oil of Guava leaves consists of a variety of active substances with broad-spectrum anti-microbial activity.The aim of this study was to extract and configure an antimicrobial solution of guava leaves volatile oil to conduct a volatile oil resistance study against three disease bacteria,SA,RS and ECCB,to reserve resources for biological control agents.The volatile oil of guava leaves was extracted by the addition of lithium salt combined with microwave-assisted hydrodistillation,the antibacterial effect of guava leaf volatile oil on three test strains was evaluated by the filter paper diffusion method and minimum inhibitory concentration(MIC),and the extraction process was optimized by single factor combined with response surface experiments.The chemical components of guava leaf volatile oil were determined by gas chromatography-mass spectrometry(GC-MS).The results showed that the oil exhibited excellent inhibitory effects against SA,RS and ECCB,with the inhibition circle diameters ranging from 20.57 to 23.24 mm and the inhibition rates reaching more than 60%,and the MIC values of 3.13μg/mL,1.56μg/mL and 3.13μg/mL,respectively.The oil had the greatest inhibitory effect on RS,the diameter of the inhibition circle was(23.24±0.47)mm,which was highly sensitive,and also had certain inhibitory effect on SA and ECCB.The optimum process conditions for the extraction process were liquid to material ratio of 10 mL/g DW,microwave power of 660 W and microwave time of 43 min.The yield of the oil obtained under the conditions was(10.23±0.17)mL/kg DW,and the validity and reproducibility of the method were verified by three parallel experiments.The components of the oil were detected to be mainly terpenes and alcohols,with relatively high content ofβ-caryophyllene(28.36%),l-
作者
张晓南
王建豪
王佳丽
朱鸿维
牛亚倩
熊婷婷
张英迪
徐福
ZHANG Xiaonan;WANG Jianhao;WANG Jiali;ZHU Hongwei;NIU Yaqian;XIONG Tingting;ZHANG Yingdi;XU Fu(Jiaying University,Meizhou,Guangdong 541000,China;College of Food Sciences,Northeast Agricultural University,Harbin,Heilongjiang 150030,China)
出处
《热带作物学报》
CSCD
北大核心
2023年第1期174-185,共12页
Chinese Journal of Tropical Crops
基金
梅州市科技专项资金项目(No.2021B0204007)。
关键词
番石榴叶
挥发油
抗菌性
响应面优化
GC-MS分析
guava leaf
volatile oil
antibacterial activity
response surface optimization
GC-MS analysis
