摘要
目的探讨姜黄素对糖尿病肾病大鼠肾脏的保护作用及可能机制。方法 36只SD大鼠随机分为对照组(control组)、糖尿病肾病模型组(DN组)和姜黄素治疗组(Cur组),每组12只。于造模成功后第8周,生化分析仪检测各组大鼠血清肌酐(Scr)与尿素氮(BUN)的含量;称量各组大鼠体重和肾重,测定肾指数;ELISA方法检测各组大鼠肾组织超氧化物歧化酶(SOD)、丙二醛(MDA)与谷胱甘肽(GSH)的表达水平;Masson染色观察肾组织纤维化的形态学变化;Western blot方法检测各组大鼠肾组织TGF-β1、p-Smad2以及Collagen I的表达变化。结果姜黄素能够降低糖尿病肾病大鼠血清中Scr、BUN含量、降低肾脏指数(P<0.05);升高SOD活性与GSH水平,降低MDA含量(P<0.05);减轻肾小球系膜基质增生程度、减少胶原纤维沉积(P<0.05);下调糖尿病肾病大鼠肾组织TGF-β1、p-Smad2与Collagen I的表达(P<0.05)。结论姜黄素可能通过抑制TGF-β1/Smad2信号通路减轻糖尿病肾病大鼠的氧化应激和肾纤维化。
Objective To investigate the protective effect of curcumin on diabetic nephropathy rats and its possible mechanism. Methods Thirty six SD rats were randomly divided into control group(control group), diabetic nephropathy model group(DN group) and curcumin treatment group(Cur group), with 12 rats in each group. At the 8 th week after successful modeling, the contents of serum creatinine(SCR) and urea nitrogen(BUN) of rats in each group were detected by biochemical analyzer;the weight and kidney weight of rats in each group were weighed, and the renal index was calculated;the levels of superoxide dismutase(SOD), malondialdehyde(MDA) and glutathione(GSH) in renal tissue of rats in each group were detected by ELISA;the morphology of renal fibrosis was observed by Masson staining. Western blot was used to detect the expression of TGF-β 1, p-Smad2 and collagen I. Results Curcumin could reduce the contents of SCR and BUN in serum and renal index of diabetic nephropathy rats(P<0.05);increase the activity of SOD and the level of GSH,reduce the content of MDA(P<0.05);reduce the proliferation of glomerular mesangial matrix, reduce the deposition of collagen fibers;down regulate the expression of TGF-β1, p-Smad2 and collagen I in renal tissue of diabetic nephropathy rats(P<0.05). Conclusion Curcumin may reduce oxidative stress and renal fibrosis in diabetic nephropathy rats by inhibiting TGF-β 1/Smad2 signaling pathway.
作者
王蕊
孔媛
吴腾飞
林淼
杨博
李兆阳
梁琳琅
WANG Rui;KONG Yuan;WU Teng-fei;LIN Miao;YANG Bo;LI Zhao-yang;LIANG Lin-lang(Department of Endocrinology,general hospital of the northern theater command,Shenyang 110016;Department of experimental animal,China Medical University,Shenyang 110122,China)
出处
《解剖科学进展》
CAS
2021年第6期748-750,754,共4页
Progress of Anatomical Sciences
基金
辽宁省教育厅科学研究一般项目(L2013318)。
