摘要
目的探究白三烯受体拮抗剂孟鲁司特对脊髓损伤大鼠的神经保护作用及可能机制。方法SD大鼠随机分为假手术组(Sham组)、脊髓损伤模型组(SCI组)和孟鲁司特(montelukast,MK)干预组(MK组);参照Allen’s方法建立脊髓损伤的大鼠模型。对大鼠进行运动功能BBB评分;HE染色观察脊髓损伤病理变化;ELISA检测脊髓组织匀浆液中IL-1β、IL-18的含量;免疫荧光方法检测小胶质细胞极化情况;Western blot法检测NLRP3、pro-caspase1、ASC的表达水平。结果白三烯受体拮抗剂孟鲁司特改善SCI大鼠病理损伤,抑制IL-1β、IL-18的释放,促进小胶质细胞由M1型向M2型转化,下调NLRP3、pro-caspase1、ASC的表达。结论白三烯受体拮抗剂孟鲁司特对SCI大鼠具有神经保护作用,其机制与抑制NLRP3炎症小体的释放调控小胶质细胞极化有关。
Objective To explore neuroprotective effect of leukotriene receptor antagonist montelukast on rats with spinal cord injury and its possible mechanism. Methods SD rats were randomly divided into sham operation group(Sham group), spinal cord injury model group(SCI group), and montelukast(montelukast, MK) intervention group(MK group);rat model of spinal cord injury was established according to Allen’s method. BBB score was used to evaluate motor function of rats. HE staining was used to observe pathological changes of spinal cord injury. ELISA was used to detect the content of IL-1β and IL-18 in spinal cord homogenate. Immunofluorescence method was used to detect microglia polarization. Western blot was used to detect NLRP3, Pro-caspase1 and ASC. Results Montelukast improved the pathological damage of SCI rats, inhibited the release of IL-1β and IL-18, promoted the transformation of microglia from M1 type to M2 type, and down-regulated the expression of NLRP3, pro-caspase1, and ASC. Conclusion Montelukast has neuroprotective effect on SCI rats, and its mechanism is related to the regulation of microglial polarization by inhibiting the release of NLRP3 inflammasome.
作者
季英楠
东黎阳
冯时
JI Ying-nan;DONG Li-yang;FENG Shi(Department of Orthopedics,Affiliated Central Hospital of Shenyang Medical College,Shenyang,110024)
出处
《解剖科学进展》
CAS
2021年第6期709-712,共4页
Progress of Anatomical Sciences
基金
沈阳医学院科技基金项目(20142035)。
关键词
孟鲁司特
脊髓损伤
NLRP3炎症小体
小胶质细胞
Leukotriene receptor antagonists
spinal cord injury
NLRP3 inflammatory body
microglia
