摘要
目的探讨姜黄素调控miR-199a-3p的基因表达对前列腺癌C4-2细胞增殖、迁移和侵袭的影响。方法将miR-199a-3p抑制物及阴性对照转染至C4-2细胞中,并分别标记为anti-miR-199a-3p组和anti-miR-con组;将仅加入脂质体的C4-2细胞标记为对照组。运用MTT法检测通过姜黄素(0、20、40、80μmol/L)处理的C4-2细胞的增殖情况。40μmol/L姜黄素处理C4-2细胞后,Transwell法检测细胞的迁移和侵袭能力;qRT-PCR检测细胞的miR-199a-3p表达量。将inhibitor NC、miR-199a-3p inhibitor转染至C4-2细胞中,再用40μmol/L姜黄素处理48 h,采用MTT法、Transwell法检测各组细胞的增殖、迁移和侵袭情况;Western blot检测各组C4-2细胞中基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)、β-catenin、Cyclin D1和c-Myc的蛋白表达量。结果与对照组比较,姜黄素能明显抑制C4-2细胞的增殖、迁移和侵袭(P<0.05)。姜黄素(40μmol/L)处理后,C4-2细胞中miR-199a-3p的表达量显著增加(P<0.05)。抑制miR-199a-3p的表达,可逆转姜黄素对C4-2细胞增殖、迁移和侵袭的抑制作用(P<0.05)。使用姜黄素处理miR-199a-3p低表达的C4-2细胞后,与anti-miR-con组相比,anti-miR-199a-3p组C4-2细胞的MMP-2、MMP-9的表达量显著增加(P<0.05),β-catenin、Cyclin D1和c-Myc蛋白表达量均显著增加(P<0.05)。结论姜黄素可上调miR-199a-3p的表达,从而抑制前列腺癌C4-2细胞的增殖、迁移和侵袭。
Objective To investigate the effect of curcumin on the proliferation,migration and invasion of C4-2 cells in prostate cancer by regulating miR-199a-3p.Methods Methyl thiazolyl tetrazolium(MTT)assay was used to detect the proliferation of C4-2 cells treated with different concentrations of curcumin(0,20,40,80μmol/L).Treatment of C4-2 cells with 40μmol/L curcumin,the migration and invasion were detected by transwell assay,and the expression of mir-199a-3p was detected by qRT-PCR.Inhibitor NC and mir-199a-3p inhibitor were transfected into C4-2 cells,and the cell was treated with 40μmol/L curcumin,the proliferation,migration and invasion of C4-2 cells in each group were detected by MTT assay and Transwell assay;the expressions of MMP-2,MMP-9,β-catenin,Cyclin D1 and c-Myc in C4-2 cells in each group were detected by Western blot.Results Curcu-min significantly inhibited the proliferation,migration and invasion of C4-2 cells(P<0.05).After curcumin treatment,miR-199a-3p expression in C4-2 cells was significantly increased(P<0.05).Inhibiting the expression of miR-199a-3p reversed the inhibitory effect of curcumin on the proliferation,migration and invasion of C4-2 cells.Conclusions Curcumin can up-regulate the expression of miR-199a-3p,thereby inhibiting the proliferation,migration and invasion of C4-2 cells in prostate cancer.
作者
李晓明
张杜平
邹铁军
Li Xiaoming;Zhang Duping;Zou Tiejun(Department of Emergency Surgery,the People's Hospital of Shaanxi Province,Xi'an 710068,China;Department of Urology,521 Hospital of Norinco Group,Xi'an 710065,China;Department of Urology,the People's Hospital of Shaanxi Province,Xi'an 710068,China)
出处
《国际泌尿系统杂志》
2022年第1期31-35,共5页
International Journal of Urology and Nephrology
