摘要
目的 检测姜黄素在体外对雄激素非依赖性前列腺癌细胞（PC-3）生物学行为的影响,并探讨其机制.方法 分别采用CFSE染色、Annexin V-PI染色和PI染色法,使用流式细胞仪分析姜黄素对PC-3细胞增殖、凋亡和细胞周期的影响.然后采用荧光素酶报告基因系统检测姜黄素在体外对核因子-κB（NF-κB）、激活蛋白-1（AP-1）和p53三个信号通路的影响.结果 50μmol/L的姜黄素作用后,PC-3细胞的增殖指数从7.08±0.20降到4.38±0.19（P＜0.05） 凋亡率从（5.34±0.96）%提高到（21.53±2.87）%（P＜0.05） G2/M期细胞比例由（34.27±1.87）%上升到（57.29±1.91）%（P＜0.05）.荧光素酶活性检测结果显示,姜黄素能在体外抑制NF-κB和AP-1信号通路活性（P＜0.05）.结论 姜黄素能在体外显著抑制雄激素非依赖性前列腺癌细胞的增殖,诱导细胞凋亡并导致细胞周期的阻滞.姜黄素可能通过抑制NF-κB和AP-1信号通路发挥抑癌效应.
Objective To investigate the anti-tumor activity and mechanisms of curcumin in androgen independent prostate cancer in vitro. Methods After curcumin treatment, the effect of curcumin on the proliferation of prostate cancer cells were assessed by CFSE dilution. Flow cytometery （ FCM ） were performed to analysis the cell cycle and the induction of apoptosis on tumor cells. A luciferase reporter gene assay was used to determine the effects of curcumin on the activities of intracellular nuclear factor-κB （NF-κB）, activator protein-1 （AP-1） and p53 signaling pathways. Results After treating with 50 μmol/Lcurcumin the proliferation index of androgen independent prostate cancer cells in vitro decreased from 7. 08 ± 0. 20 to 4. 38 ± 0. 19 （ P 〈 0. 05 ） the percentage of apoptotic cells increased from （ 5.34 ±0.96）% to （21.53 ±2.87）% （P〈0. 05） and the percentage of G2/M phase cells increased from （34. 27 ± 1.87） % up to （57.29 ± 1.91 ） % （P 〈 0. 05 ）. The results of the luciferase assay showed that curcumin selectively inhibited the activities of the NF-κB and AP-1 signaling pathway in PC-3 cells significantly. Conclusion Curcumin could significantly inhibit the androgen independent prostate cancer through blocking cellular proliferation and inducing apoptosis. Inhibition of transcription factors NF-κB and AP-1 contribute to the in vitro anti-tumor activity of curcumin.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第11期1711-1713,共3页
Chinese Journal of Experimental Surgery
