摘要
目的探究参苓白术散对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)模型小鼠的疗效。方法选用雄性BALB/c小鼠(6~8周龄,18~20 g)36只,随机分成6组,每组6只。A组:参苓白术散高剂量组;B组:参苓白术散中剂量组;C组:参苓白术散低剂量组;D组:柳氮磺胺吡啶治疗组;E组:空白对照组;F组:模型组。以连续7 d自由饮用3.5%葡聚糖硫酸钠水溶液建立急性UC模型,造模同时分别灌胃给予不同药物治疗,观察记录小鼠临床表现并结合疾病活动指数评分(DAI)以及处死后的结直肠长度、结肠黏膜损伤眼观评分、结肠组织病理学评分等综合评价参苓白术散对UC模型小鼠的治疗效果。结果与E组比较,F组小鼠DAI评分显著升高,结肠明显缩短,结肠黏膜损伤眼观评分及组织病理学评分显著升高。B组小鼠的病情改善优于A、C、D组。结肠黏膜损伤评分显示,F组与B组比较差异有显著性(P<0.05)。参苓白术散可以改善小鼠结肠组织局部损伤,缓解小鼠UC的症状。结论参苓白术散对UC模型小鼠有良好的治疗效果,参苓白术散中剂量的疗效优于参苓白术散高剂量和低剂量。
Objective To explore the curative effect of Shenling Baizhu powder on dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)model mice.Methods Thirty-six male BALB/c mice(6~8 weeks,18~20 g)were randomly divided into 6 groups with 6 mice in each group:group A,high-dose Shenling Baizhu powder;group B,middle-dose Shenling Baizhu powder;group C,low-dose Shenling Baizhu powder;group D,sulfasalazine treatment;group E,blank control;and group F,model.The acute UC model was established by administering 3.5%dextran sulfate sodium solution with drinking water for 7 days,and at the same time of modeling,different drugs were given by intragastric administration.The therapeutic effect of Shenling Baizhu powder on UC mice was evaluated by assessing their clinical manifestations,disease activity index(DAI),colon and rectum length,colon mucosa injury and colon histopathology.Results Compared with group E,the DAI score was evidently higher,the colon was markedly shorter,and the visual score and histopathology score of colonic mucosa injury were significantly higher in group F.The condition of group B was better than that of groups A,C and D.The score of colonic mucosal injury in group F was distinctly higher than that in group B(P<0.05).Shenling Baizhu powder improved the local injury of colon tissue and relieved the symptoms of UC.Conclusions Shenling Baizhu powder has a good therapeutic effect on UC model mice,and the curative effect of a middle dose of Shenling Baizhu powder was better than that of the high and low doses of Shenling Baizhu powder.
作者
马琪
翁与竞
李佳
覃施媛
何燕凤
马旭东
MA Qi;WENG Yujing;LI Jia;QIN Shiyuan;HE Yanfeng;MA Xudong(College of Veterinary Medicine,Southwest University,Chongqing 402460,China;Immunology Research Center,Medical Research Institute,Southwest University,Chongqing 402460)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2021年第6期785-792,共8页
Acta Laboratorium Animalis Scientia Sinica
基金
西南大学新进博士科研资助金(5360300213)
重庆市自然科学基金(cstc2021jcyj-msxmX0982)。
关键词
溃疡性结肠炎
参苓白术散
葡聚糖硫酸钠
ulcerative colitis
Shenling Baizhu powder
sodium dextran sulfate
