摘要
用葡聚糖硫酸钠盐(DSS)构建昆明小鼠溃疡性结肠炎(UC)模型并筛选建模最佳浓度。32只雄性昆明小鼠分成4组(空白组、30、40、50 g/L DSS组),每组8只。各组小鼠分别自由饮用7 d无菌水或不同浓度DSS,每天记录小鼠体重,观察腹泻及便血情况,计算疾病活动指数。造模结束后测量结肠长度并观察结肠组织形态。结果表明,不同浓度的DSS可显著提高小鼠的疾病活动指数,并以剂量依赖的方式诱发小鼠的体重减轻、腹泻和便血症状。与空白组相比,不同浓度DSS均破坏了结肠组织形态,但30 g/L DSS组小鼠结肠炎症发生程度较轻,而40 g/L DSS组与50 g/L DSS组小鼠结肠炎性病变严重,表现出明显的急性UC的特征。由于50 g/L DSS组小鼠精神状态不佳,故选择40 g/L DSS作为昆明小鼠造模最适浓度。研究结果表明自由饮用7 d 40 g/L DSS可以成功建立昆明小鼠UC模型,诱使其出现腹泻、体重下降、便血等疾病活动状态,表现出黏膜糜烂、炎症浸润等急性UC病理组织学特征。研究结果可为UC的药物研发及机制研究动物模型建立提供参考。
Ulcerative colitis(UC)model of Kunming mice was established by dextran sodium sulfate(DSS)and the optimal concentration was selected.Thirty-two male Kunming mice were randomly divided into four groups:control group,30 g/L DSS group,40 g/L DSS group and 50 g/L DSS group,with 8 mice in each group.Mice in each group were free to drink sterile water or DSS with different concentrations for 7 days.Then the weight,diarrhea and hematochezia were recorded every day,and disease activity index was calculated.After modeling,the length of colon was measured and the morphology of colon tissue was observed.The results showed that the disease activity index was significantly increased in mice treated with different concentrations of DSS.In addition,the changes in weight,diarrhea and hematochezia were in a dose-dependent manner.Compared with the control group,the colon tissue was damaged by different concentrations of DSS,but the inflammation of colon was mild in mice treated with 30 g/L DSS group,while the colon lesions of mice in the 40 g/L DSS and 50 g/L DSS groups were serious,showing obvious characteristics of acute UC.Due to the poor mental state of mice in the 50 g/L DSS group,40 g/L DSS was the optimal concentration for Kunming mice.This study suggested that UC model of Kunming mice could be successfully established using 40 g/L DSS for 7 days.The model mice showed diarrhea,weight loss,hematochezia and other disease activities,and the histopathological features of UC such as mucosal erosion and inflammatory infiltration were observed obviously.The results could provide the basis of animal model for further drug development and mechanism study of UC.
作者
杨阳
周协琛
李涛
李炎
曹俊阳
赵蕊
YANG Yang;ZHOU Xie-chen;LI Tao;LI Yan;CAO Jun-yang;Zhao Rui(Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang,163319,China)
出处
《动物医学进展》
北大核心
2023年第2期72-77,共6页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(31772789)
黑龙江省大学生创新创业培训项目(201810223005)
黑龙江八一农垦大学研究生创新项目(YJSCX2021-Y103)。
