摘要
溃疡性结肠炎是目前研究的重点疾病。基础研究所用溃疡性结肠炎模型成为其研究的首要关键问题,模型的成功建立是决定实验成功的根本。目前基础研究所用的小鼠模型建立方法最常用为化学法诱导,大鼠模型建立最常用的方法有化学法、免疫复合法及复合法等。其中,化学法诱导的模型,所用的剂量大小是成功建立溃疡性结肠炎模型的关键。根据实验报道3.5%的葡聚糖硫酸钠(DSS)灌胃诱导的小鼠模型最为合适,而50 mg·kg^(-1)TNBS/40%乙醇灌肠量诱导的大鼠模型最为适合。DSS建立的模型操作简单,目前所用DSS诱导建立大鼠模型的量有待进一步探讨。免疫复合法建立的大鼠模型优于单纯的免疫法所建立的模型,且溃疡性结肠炎模型病理特点持续时间长达1~2个月之久。复合法建立的大鼠模型成功率高,病程持久,是一种理想的溃疡性结肠炎模型。
The ulcerative colitis is the focus study disease in recent years.The establishment of the ulcerative colitis model adopted by the fundamental study is a key question,which determines the success of the whole experiment.At present,the establishment method of mouse model used in basic research is most commonly induced by chemical method.The basic study of rat model establishment method has the chemical method,the complex immune legal,the complex legal.etc.Among the chemical method,the dose size is the key to the successful establishment of ulcerative colitis model,according to the experiment reported that the experimental mouse model induced by3.5% DSS lavage is the most appropriate and the 50 mg·kg（-1） drug dosage,the TNBS/alcohol 40%,is the most suitable. The model operation of DSS solution is simple,and the amount of DSS induced establishment of rat model should be further explored.The rat model established by immune complex is better than the model established by the simple immune method,and the pathological characteristics of ulcerative colitis model lasting for 1-2 months. The complex legal model has the advantage of the high success rate,long lasting,is an ideal model of ulcerative colitis.
作者
崔国宁
刘喜平
董俊刚
曾庆涛
CUI Guoning;LIU Xiping;DONG Jungang;ZENG Qingtao(Gansu University of Chinese Medicine, Lanzhou, Gansu 730000, Chin)
出处
《今日药学》
CAS
2018年第4期280-284,共5页
Pharmacy Today
基金
国家自然科学基金项目(81560747)
