摘要
目的研究日光紫外线暴露对皮肤的损害作用。方法设日光紫外线非暴露组、低暴露组和高暴露组,日光紫外线照射剂量分别为0、10 J/cm^(2)和20 J/cm^(2),每组10只小鼠。采用日光紫外线模拟器模拟日光紫外线,照射小鼠皮肤,每周连续照射5 d,持续照射13周。实验期末检查小鼠皮肤外观,检测皮肤水分、油分、纹理密度、羟脯氨酸(HYP)、透明质酸(HA)、丙二醛(MDA)、谷胱甘肽(GSH)和超氧化歧化酶(SOD)活性,观察皮肤组织形态、胶原纤维形态和弹性纤维形态。结果非暴露组小鼠皮肤外观正常;低暴露组仅1只小鼠皮肤轻度脱屑;高暴露组小鼠皮肤外观松弛起皱,干燥脱屑,局部增厚伴红斑形成。与非暴露组比较,高暴露组小鼠皮肤水分、HYP和HA含量以及SOD活性均较低(P<0.05);纹理密度,MDA含量,皮肤组织、胶原纤维组织和弹性纤维组织形态学得分均较高(P<0.05)。与低暴露组比较,高暴露组小鼠HA含量和SOD活性较低(P<0.05);皮肤纹理密度,MDA含量,以及皮肤组织和胶原纤维组织形态学得分均较高(P<0.05)。结论20 J/cm^(2)的日光紫外线暴露可明显导致小鼠皮肤外观和功能异常。
Objective To study the damage effect of sunlight ultraviolet exposure on skin.Methods No exposure group,low exposure group and high exposure group were set up with 10 mice in each.The exposure doses of sunlight ultraviolet were 0,10 J/cm^(2) and 20 J/cm^(2),respectively.The skin of mice was irradiated by a sunlight ultraviolet simulator for 5 days a week,13 weeks.At the end of the experiment,the skin appearance of mice was examined;the skin moisture,oil content,texture density,hydroxyproline(HYP),hyaluronic acid(HA),malondialdehyde(MDA),glutathione(GSH)and superoxide dismutase(SOD)activities were detected;and the skin tissue morphology,collagen fiber morphology and elastic fiber morphology were observed.Results The skin appearance of mice in the no exposure group was normal;in the low exposure group,only one mouse had mild skin desquamation;in the high exposure group,the skin was loose and wrinkled,dry and desquamated,local thickening and erythema formation.Compared with the no exposure group,the contents of skin moisture,HYP,HA and SOD activity were lower,texture density,MDA content,morphological scores of skin tissue,collagen fiber tissue and elastic fiber tissue were higher in the high exposure group(all P<0.05).Compared with the low exposure group,the HA content and SOD activity were lower,the skin texture density,MDA content,and histomorphological scores of skin tissue and collagen fibers were higher in the high exposure group(all P<0.05).Conclusion Exposure to 20 J/cm^(2) sunlight ultraviolet can significantly lead to abnormal skin appearance and function in mice.
作者
夏勇
宋燕华
徐彩菊
鹿伟
严峻
蔡德雷
谢佳莹
XIA Yong;SONG Yanhua;XU Caiju;LU Wei;YAN Jun;CAI Delei;XIE Jiaying(Department of Physicochemical and Toxicology,Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang 310051,China)
出处
《预防医学》
2022年第1期95-100,共6页
CHINA PREVENTIVE MEDICINE JOURNAL
关键词
日光紫外线
亚慢性暴露
皮肤损害
sunlight ultraviolet
subchronic exposure
skin damage
