摘要
自抱卵的中华锯齿米虾雌虾腹部剥离取卵裂期、囊胚期、原肠胚期和无节幼体Ⅰ期的胚胎置于培养皿中,暴露在紫外线下,照射剂量分别为0、1、2、4、8、16 W·s/cm^2,人工振荡培养箱中以速度55 r/min、温度25℃、湿度60%培养。每隔24 h在解剖镜下观察胚胎发育至48 h后进行数据分析,研究紫外线照射剂量对中华锯齿米虾胚胎发育的影响。结果表明,胚胎的存活率对紫外线具有明显的剂量依赖关系。随着紫外线照射剂量的增加,胚胎发育延缓。紫外线暴露后48 h的半数致死量分别为:卵裂期11.5 W·s/cm^2、囊胚期8.6 W·s/cm^2、原肠胚期12.0 W·s/cm^2、无节幼体Ⅰ期14.2 W·s/cm^2。相同的暴露剂量下,暴露时间对胚胎发育也有明显影响。
The embryos of Chinese freshwater prawn Neocaridina denticulata sinensis were detached in petri dishes from the abdomen at stages of cleavage,blastula,gastrula and nauplius larvaⅠand exposed to ultraviolet radiation(UV)at doses of 0 W·s/cm^2,1 W·s/cm^2,2 W·s/cm^2,4 W·s/cm^2,8 W·s/cm^2 and 16 W·s/cm^2 in a manual shaking incubator at a speed of 55 r/min,a temperature of 25℃,and a humidity of 60%.The embryonic development was observed under a dissecting scope every 24 hours,and data analysis was performed after 48 h to investigate the effect of UV dose on the embryonic development of Chinese freshwater prawn.The results showed that Chinese freshwater prawn had the survival rate of the embryo in a dose-dependent relationship with UV dose exposure with severely delayed embryo developmen with the increase in UV dose.The median lethal dose at the 48 h was found to be 11.5 W·s/cm^2 in cleavage stage,8.6 W·s/cm^2 in blastula stage,12.0 W·s/cm^2 in gastrula stage,and 14.2 W·s/cm^2 in nauplius I stage.In addition,exposure period showed significant impact on embryonic development.
作者
宋紫玉
王国栋
张丽莉
黄世玉
洪伟
贾奔
张元长
SONG Ziyu;WANG Guodong;ZHANG Lili;HUANG Shiyu;HONG Wei;JIA Ben;ZHANG Yuanchang(College of Fisheries,Jimei University,Xiamen 361021,China)
出处
《水产科学》
CAS
CSCD
北大核心
2021年第1期116-121,共6页
Fisheries Science
基金
国家自然科学基金资助项目(31702339)
福建省科技计划项目(2018I0013)
福建省种业创新与产业化工程项目(2017FJSCZY02).
关键词
中华锯齿米虾
紫外线
胚胎发育
半数致死量
Neocaridina denticulata sinensis
ultraviolet ray
embryogenesis
median lethal dose
