摘要
目的通过研究应用不足量全血制备去白细胞悬浮红细胞的可行性,并建立制备方法,减少血液报废,提高血液的利用率,增加血站的血液供应。方法将南海血站采集的不足量全血,按采集后至成分制备时的保存时间不同随机分成4组,全部经成分制备成去白细胞悬浮红细胞,保存后进行质检,统计分析质检结果。同期足量采血的全血作为对照组。结果实验组共有177袋不足量全血制备成去白细胞悬浮红细胞,其中制备成0.5 U的56袋、1.0 U的80袋、1.5 U的41袋,全部血液的外观、容量和血红蛋白水平、血细胞比容、白细胞残留量、储存期末溶血率、无菌试验均合格,符合《全血及成分血质量要求(GB18469-2012)》标准;对照组的40袋血液质检结果全部合格;实验组与对照组制备的去白细胞悬浮红细胞质检结果比较差异无统计学意义(P>0.05)。结论不足量全血制备去白细胞悬浮红细胞具备可行性,其血液质量符合国家标准,值得推广。
Objective To establish a preparation method of preparing leukocyte-depleted suspension red blood cells from whole blood with insufficient volume to reduce the waste of blood, improve the utilization rate of blood and increase the blood supply.Methods The whole blood with insufficient volume collected from Nanhai Blood Station was randomly divided into 4 groups according to the storage time from collection to preparation of the components, and was prepared into leukocyte-depleted suspension red blood cells.Quality inspection and statistical analysis were conducted subsequently.Whole blood with sufficient volume collected at the same time was set as the control group.Results In the experimental group altogether 177 bags of whole blood with insufficient volume were prepared into leukocyte-depleted suspension red blood cells, among which 56 bags were 0.5 U/bag, 80 bags were 1.0 U/bag and 41 bags were 1.5 U/bag.The appearance, volume, hemoglobin content, hematocrit, leucocyte residual quantity, hemolysis rate at the end of storage period and sterility test of them all were qualified, and met the standard of whole blood and blood component quality requirement(GB18469-2012).There was no statistical significance in the quality inspection results of leukocyte-depleted suspension red blood cells prepared between the experimental group and the control group(P>0.05).Conclusion The preparation of leukocyte-depleted suspension red blood cells from whole blood with insufficient volume is feasible and worth popularizing.The blood quality met the national standard.
作者
杨图深
刘小华
谭雄锐
陈锦艳
YANG Tushen;LIU Xiaohua;TAN Xiongrui;CHEN Jinyan(Nanhai Blood Station of Foshan Blood Center,Foshan,Guangdong 528200,China;Guangzhou Blood Center,Guangzhou,Guangdong 510180,China)
出处
《检验医学与临床》
CAS
2022年第1期23-25,30,共4页
Laboratory Medicine and Clinic
基金
佛山市科学技术研究项目(2018AB001282)。
关键词
不足量
全血
制备
去白细胞悬浮红细胞
insufficient volume
whole blood
preparation
leukocyte-depleted suspension red blood cells
