摘要
研究芩暴红止咳口服液抗炎、止咳、祛痰和平喘的药理作用,并通过网络药理学探究其作用机制。采用二甲苯致小鼠耳廓肿胀实验观察抗炎作用;小鼠氨水引咳法观察止咳作用;小鼠气管酚红排泌法观察祛痰作用;磷酸组胺和氯化乙酰胆碱联合致小鼠哮喘观察平喘作用;采用TCMSP数据库及文献查找芩暴红止咳口服液各中药化学成分,利用BATMAN-TCM进行成分靶点预测。通过GeneCards数据库查询炎症、咳嗽、痰、哮喘相关疾病靶点。药物与疾病靶点相交集后,分别利用Metascape数据库进行GO生物过程分析和KEGG通路富集分析,并导入STRING数据库检索蛋白互作关系,筛选关键靶点。结果显示,芩暴红止咳口服液4.5、9.0 mL·kg^(-1)可显著降低右耳质量(P<0.05)和厚度(P<0.01),且减少两耳质量差(肿胀度)(P<0.05),明显延长咳嗽潜伏期(P<0.05),显著降低3 min内咳嗽次数(P<0.05),显著增加气管酚红排泌量(P<0.01);芩暴红止咳口服液2.3、4.5、9.0 mL·kg^(-1)可明显延长引喘潜伏期(P<0.05)。芩暴红止咳口服液共324个化学成分,1245个靶点,炎症、咳嗽、痰、平喘靶点分别为10272、4400、192、7533个,交集后,芩暴红止咳口服液抗炎、止咳、祛痰、平喘共同涉及调节离子运输、血液循环等GO生物过程和癌症相关信号通路、神经活性配体-受体相互作用等KEGG通路。通过实验验证和网络药理学分析,均证明芩暴红止咳口服液具有较好的抗炎、止咳、祛痰和平喘作用。
This study aimed to investigate the anti-inflammatory,antitussive,expectorant,and anti-asthmatic effects of Qinbaohong Oral Liquid in mouse experiments and explore its action mechanism based on network pharmacology.The mouse auricle swelling was induced by xylene for detecting the anti-inflammatory effect of Qinbaohong Oral Liquid,whose antitussive effect was then examined in mice with cough after exposure to ammonium hydroxide.The expectorant effect was determined based on the excretion of phenol red into the mouse trachea.The mouse model of asthma induced by histamine phosphate and acetylcholine chloride was used to observe the antiasthmatic effect.The chemical components of Qinbaohong Oral Liquid were retrieved from TCMSP and literature,followed by target prediction based on BATMAN-TCM.The targets of inflammation,cough,expectoration,and asthma collected from GeneCards were intersected with drug targets for GO and KEGG enrichment analysis using Metascape.The results were imported into STRING for exploring protein-protein interactions and screening the key targets.As demonstrated by our findings,Qinbaohong Oral Liquid at 4.5 and 9.0 mL·kg^(-1) obviously decreased the weight(P<0.05)and thickness(P<0.01)of the right swelling ear and also the weight difference(swelling degree)between the two ears(P<0.05),prolonged the incubation period of cough(P<0.05),reduced the frequency of cough within 3 min(P<0.05),and increased the excretion of phenol red into the mouse trachea(P<0.01).Qinbaohong Oral Liquid at 2.3,4.5,and 9.0 mL·kg^(-1) dramatically prolonged the incubation period of asthma(P<0.05).A total of 324 chemical components and 1245 targets were harvested for the Qinbaohong Oral Liquid,together with 10272 inflammation targets,4400 cough targets,192 expectoration targets,and 7533 asthma targets.Their intersection revealed that the anti-inflammatory,antitussive,expectorant and anti-asthmatic effects of Qinbaohong Oral Liquid were correlated with such GO biological processes as the regulation of ion transport and blo
作者
李煜
张毅
徐核
王丽芳
张方博
杨洪军
LI Yu;ZHANG Yi;XU He;WANG Li-fang;ZHANG Fang-bo;YANG Hong-jun(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第22期5902-5911,共10页
China Journal of Chinese Materia Medica
基金
国家“重大新药创制”科技重大专项(2019ZX09201005)。
关键词
芩暴红止咳口服液
抗炎
止咳
祛痰
平喘
网络药理学
作用机制
Qinbaohong Oral Liquid
anti-inflammation
stopping cough
eliminating phlegm
relieving asthma
network pharmacology
mechanism
