摘要
目的:建立测定暴马子皮药材中2个成分(紫丁香苷、sinapylaldehyde 4-O-β-D-glucopyranoside)的高效液相色谱分析方法。方法:采用Phenomenex Luna C18(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇(B)-0.1%醋酸水溶液(A),梯度洗脱,流速1.0 mL·min-1,DAD检测器,检测波长:284 nm;柱温25℃。结果:紫丁香苷、sinapylaldehyde 4-O-β-D-gluco-pyranoside的线性范围分别为0.2～0.8μg(r=0.9995)和0.04～0.16μg(r=0.9993),平均加样回收率(n=6)分别为99.9%(RSD=2.1%)和99.5%(RSD=2.9%)。结论:不同产地暴马子皮药材中所含紫丁香苷、sinapylaldehyde 4-O-β-D-gluco-pyranoside的含量存在显著性差异。本方法操作简便,结果准确,重复性好,可用于中药暴马子皮药材的质量控制。
Objective：To develop an HPLC method for the determination of syringin and sinapyl aldehyde 4-O-β-D-glucopyranoside in Syringae Cortex.Methods：The separation was performed on a Phenomenex Luna C18（4.6 mm×25 0 mm,5 μm） column.The mobile phase was composed of methanol and water solvent containing 0.1% acetic acid with gradient elution.The flow rate was 1.0 mL·min-1 and the detection wavelength was 284 nm.The column temperature was set at 25 ℃.Results：The calibration curves were linear in the ranges of 0.2-0.8 μg for syringin（r=0.9995） and 0.04-0.16 μg for sinapyl aldehyde 4-O-β-D-glucopyranoside（r=0.9993）.The average recoveries（n=6） of syringin and sinapyl aldehyde 4-O-β-D-glucopyranoside were 99.9%（RSD=2.1%） and 99.5%（RSD=2.9%）,respectively.Conclusion：The yields of the two components in different origins exhibit significant differences.This method is simple,accurate and repeatable,which can be used for the quality control of Syringae Cortex.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第10期1858-1860,1869,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金资助项目(编号:30873356
81173506)
