摘要
目的:建立HPLC法测定王不留行中王不留行环肽A和王不留行环肽B含量。方法:采用超声辅助提取王不留行环肽A和王不留行环肽B,选用Agilent C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水为流动相进行梯度洗脱,流速为1.0 mL·min-1,检测波长为220 nm。结果:王不留行环肽A和王不留行环肽B进样浓度分别在2.57～12.85μg·mL-1和0.95～13.54μg·mL-1范围内线性关系良好(r≥0.9996);平均加样回收率(n=6)分别为97.1%和95.9%,RSD分别为1.3%和1.7%。结论:该方法简便、准确,重复性好,可用于王不留行中王不留行环肽A和王不留行环肽B的含量测定。
Objective：To establish an HPLC method for the determination of segetalin A and segetalin B in Vaccariae Semen.Methods： Analysis was carried out on an Agilent C18（250 mm×4.6 mm,5 μm） column with acetonitrile-water as mobile phase in gradient elution.The flow rate was 1.0 mL·min-1 and detective wavelength was 220 nm.Results： The linear ranges of segetalin A and segetalin B were 2.57-12.85 μg·mL-1 and 0.95-13.54 μg·mL-1（r≥0.9996）,respectively.The average recoveries were 97.1% and 95.9% with RSDs of 1.3% and 1.7%,respectively.Conclusion： The results show that the method is simple,accurate and repeatable,and can be used for the quantitative analysis of segetalin A and segetalin B in Vaccariae Semen.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第5期793-796,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金项目(20872083)
"重大新药创制"科技重大专项(2010ZX09401-302-5-12)**
