摘要
目的探讨早产儿肠道微生态变化及其与胎龄、出生体质量的关系。方法选取2018年5月至2020年5月我院收治的80例早产儿作为早产组,同期收治的80例足月新生儿作为对照组。收集入组新生儿出生后3d、3周的粪便,比较2组研究对象粪便标本中细菌丰富度和Shannon-Wiener指数;根据早产组胎龄和出生体质量不同分组,分析胎龄、出生体质量与肠道微生态变化的关系。结果(1)出生3d,早产组新生儿粪便标本的DGGE图谱条带数、Shannon-Wiener指数均显著低于对照组(t=3.179、3.521,均P<0.05);(2)出生3d,胎龄34+1~36+6周组新生儿粪便标本的DGGE图谱条带数、Shannon-Wiener指数显著高于28~30周组、30+1~32周组和32+1~34周组(q=9.653、6.476、4.848和8.796、8.008、6.277,均P<0.05);(3)出生3d,体质量>2000g组新生儿粪便标本的的DGGE图谱条带数、Shannon-Wiener指数显著高于≤1500g组(q=5.601和4.593,均P<0.05);(4)不同出生体质量、胎龄的早产儿,出生3周粪便标本的DGGE图谱条带数、Shannon-Wiener指数相比差异无统计学意义(F=1.577和2.326,均P>0.05)。结论与足月新生儿相比,早产儿出生后细菌定植出现延迟且多样性差,胎龄越小、出生体质量越低的早产儿的肠道菌群的多样性更低、定植延迟的可能性更大,出生3周后其肠道微生态逐渐恢复正常。
Objective To explore the changes of intestinal microecology and their relationship with gestational age and birth weight in premature infants.Methods Eighty premature infants admitted to the hospital were enrolled as premature delivery group between May 2018and May 2020,while other 80full-term neonates during the same period were enrolled as control group.Their feces at 3dand 3weeks after birth were collected to compare bacterial abundance and Shannon-Wiener index between the two groups.The infants in premature delivery group were further grouped according to different gestational age and birth weight.The relationship between gestational age,birth weight and changes of intestinal microecology was analyzed.Results(1)At 3dafter birth,number of DGGE bands in neonatal feces specimens and Shannon-Wiener index in premature delivery group were significantly lower than those in control group respectively(t=3.179,t=3.521,all P<0.05).(2)At 3dafter birth,number of DGGE bands in neonatal feces specimens and Shannon-Wiener index in 34+1-36+6 week group were significantly higher than those in 28-30week group,30+1-32week group and 32+1-34week group(q=9.653,q=6.476,q=4.848;q=8.796,q=8.008,q=6.277,all P<0.05).(3)At 3dafter birth,number of DGGE bands in neonatal feces specimens and Shannon-Wiener index in>2000g group were significant higher than those in≤1500g group(q=5.60,q=14.593,all P<0.05).(4)There was no significant difference in number of DGGE bands in neonatal feces specimens or Shannon-Wiener index at 3weeks after birth among premature infants with different birth weight and gestational age(F=1.577,F=2.326,all P>0.05).Conclusion Compared with fullterm neonates,there are delayed colonization and poor diversity in premature infants after birth.The lower the gestational age and birth weight,the higher the possibility of lower intestinal flora diversity and delayed colonization.At 3weeks after birth,the intestinal microecology returns to normal gradually.
作者
丁再萌
祖婷
DING Zai-meng;ZU Ting(Xuzhou Maternal and Child Health Hospital,Xuzhou,Jiangsu 221000,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
2021年第11期1264-1268,共5页
Chinese Journal of Microecology
基金
江苏省妇幼健康项目(F201552)。
关键词
肠道
微生态
胎龄
早产儿
出生体质量
Intestinal tract
Microecology
Gestational age
Premature infant
Birth weight
