摘要
In the present study,we combined CaCO_(3)NPs and Ce6 to construct CaCO_(3)-Ce6 nanoparticles (NPs).CaCO_(3)-Ce6 NPs were characterized in terms of particle size,zeta potential,UV-Vis absorption spectrum,fluorescence spectrum,FTIR spectrum,and pH-responsive behavior.The reactive oxygen species (ROS) generation in vitro was measured in 4T1 cells.The results showed that CaCO_(3)-Ce6 NPs were uniform-sized NPs with excellent fluorescence properties and pH-responsive behavior.The ability of ROS generation by CaCO_(3)-Ce6 NPs was stronger compared with Ce6 in 4T1 cells because Ca;could enhance the ROS generation,which could contribute to a stronger anti-tumor effect.
本研究将碳酸钙纳米粒与光敏剂二氢卟吩e6(Ce6)结合构建碳酸钙-二氢卟吩e6纳米粒(CaCO_(3)-Ce6 NPs),对其粒径、电位、光学性质、pH敏感性和ROS产生能力进行表征。结果表明,CaCO_(3)-Ce6 NPs为粒径均匀的纳米粒,具有优良的光学特性和pH敏感性。在4T1细胞中,660 nm激光照射下,CaCO_(3)-Ce6 NPs具有比Ce6更强的ROS产生能力。上述结果表明,CaCO_(3)-Ce6 NPs在660 nm激光照射下具有增强的光动力作用,有望获得更强的抗肿瘤药效。
