摘要
目的:研究白介素(IL)-6、C反应蛋白(CRP)在高脂喂养联合球囊损伤所致动脉粥样硬化(AS)中的表达。方法:22只新西兰大白兔被随机均分为正常对照组(接受常规饲养与假手术)和高脂组(接受高脂饲养与右髂动脉球囊剥脱术),两组均喂养12周。观察比较两组喂养前后血管超声检查指标、血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、CRP、IL-6水平、血管内膜中膜厚度比值及CRP表达面积。结果:与正常对照组比较,高脂组血管内膜-中层厚度[0.240(0.220,0.260)mm比1.010(0.970,1.380)mm]、血流速度[15.260(12.240,48.220)cm/s比69.710(57.930,80.270)cm/s]、血清TG[(0.87±0.27)mmol/L比(29.96±9.80)mmol/L]、TC[(1.71±0.65)mmol/L比(69.79±18.25)mmol/L]、LDL-C[(1.06±0.51)mmol/L比(30.48±6.14)mmol/L]、CRP[2.870(2.670,3.230)μg/ml比4.860(4.340,4.900)μg/ml]、IL-6[(21.61±8.59)pg/ml比(97.41±18.42)pg/ml]水平、血管内膜/中膜厚度比值[(0.21±0.08)比(1.86±0.56)]、内膜及中膜CRP表达面积[(0.02±0.01)比(139.09±33.72)]均显著升高,管腔内径[(1.67±0.08)mm比(0.87±0.56)mm]显著降低(P均=0.001)。HE染色结果显示:高脂组血管内膜损伤显著,部分内膜剥落,新生斑块形成,损伤内膜下可见炎性细胞聚集;正常对照组内膜光滑、完整、无增厚、平滑肌细胞层排列整齐。结论:在高脂饮食联合球囊损伤建立的AS模型中血清IL-6、CRP水平显著升高,CRP表达面积显著增大。
Objective:To study expression of interleukin(IL)-6 and C reactive protein(CRP)in atherosclerosis(AS)caused by high-fat feeding combined balloon injury.Methods:A total of 22 New Zealand white rabbits were randomly and equally divided into normal control group(received routine feeding and sham operation)and high-fat group(received high-fat feeding and right iliac artery balloon ablation).Both groups were fed for 12 weeks.Vascular ultrasound indexes,serum levels of triglyceride(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),CRP and IL-6,vascular intima/media thickness ratio and CRP expression area were observed and compared between two groups before and after feeding.Results:Compared with normal control group,there were significant rise in vascular intima-media thickness[0.240(0.220,0.260)mm vs.1.010(0.970,1.380)mm],blood flow velocity[15.260(12.240,48.220)cm/s vs.69.710(57.930,80.270)cm/s],serum levels of TG[(0.87±0.27)mmol/L vs.(29.96±9.80)mmol/L],TC[(1.71±0.65)mmol/L vs.(69.79±18.25)mmol/L],LDL-C[(1.06±0.51)mmol/L vs.(30.48±6.14)mmol/L],CRP[2.870(2.670,3.230)μg/ml vs.4.860(4.340,4.900)μg/ml]and IL-6[(21.61±8.59)pg/ml vs.(97.41±18.42)pg/ml],vascular intima/media thickness ratio[(0.21±0.08)vs.(1.86±0.56)]and CRP expression area[(0.02±0.01)vs.(139.09±33.72)]in vascular intima and media,and significant reduction in lumen diameter[(1.67±0.08)mm vs.(0.87±0.56)mm]in high-fat group(P=0.001 all).HE staining indicated that vascular intima in high-fat group was significantly damaged,part of intima was peeled off,new plaques were formed,and inflammatory cell aggregation can be found under injured intima;but intima of normal control group was smooth,intact without thickening,and smooth muscle cell layers were neatly arranged.Conclusion:Serum levels of IL-6,CRP and CRP expression area significantly increase in AS model established by high-fat feeding combined balloon injury.
作者
于丽娜
耿巍
胡莹
邢颖
李静
刘伟
YU Li-na;Hu Ying;Xing Ying;Li Jing;Liu Wei(Department of Cardiology,First Central Hospital of Baoding City,Baoding,Hebei,071000,China)
出处
《心血管康复医学杂志》
CAS
2021年第6期649-653,共5页
Chinese Journal of Cardiovascular Rehabilitation Medicine
基金
保定市科学技术计划项目(1941ZF043)。
