摘要
采用PacBio SMART技术并基于三代和二代数据(3+2策略)完成紫芝栽培品种‘武芝2号’(原名:S2)(通称‘紫芝S2’)基因组De novo测序、组装,完整地获得紫芝栽培品种‘武芝2号’(原名:S2)全基因组,序列总长度56.849 Mb,包括核基因组和线粒体基因组共72条Scaffolds。其中,核基因组16681个基因,完成其中13161个基因的注释,约占总数的78.89%;预测分析到4组串联重复的rRNA簇、405个非编码RNA和3305个启动子,并找到rpb2、beta-tubulin和18S,28S rRNA(SSU,LSU)等基因序列以及漆酶同工酶基因,鲨烯合酶、羊毛甾醇合酶基因与麦角甾醇合成代谢相关基因及其分布位置,为今后紫芝栽培品种(系)分子鉴定与菌株鉴别、功能基因的挖掘与利用提供可靠信息,同时也为其后续开展中国栽培灵芝种类的比较基因组学分析积累科学数据。
The cultivar‘Wuzhi No.2(original name:S2)’has been applied and extended in south China(Fujian,Guangdong and Guangxi province,etc.),known as Ganoderma sp.strain Zizhi S2 that belongs to Ganoderma sinense complex.Whole genome of the Zizhi S2 was completed after genomic sequencing and assembly using the PacBio SMRT technology and based on the third-generation and second-generation data(3+2 strategy),the total length of the sequence was 56.849 Mb,totally 72 scaffolds including nuclear genome and mitochondrial genome.There were 16681 genes in the nuclear genome,and 13161 genes were annotated and accounting for 78.89%of the total.Four groups of tandem repeat rRNA clusters,405 non-coding RNAs and 3305 promoters were predicted and analyzed.The gene sequences of rpb2,beta-tubulin and 18S,28S rRNA(SSU,LSU),laccase isoenzyme genes,squalene synthase and lanosterol synthesis genes,ergo-sterol synthesis and metabolism related genes and their distribution positions were revealed.This finding not only provides reliable information for the molecular identification and strain identification of cultivated varieties(lines)of Zizhi,but also for further mining and utilization of functional genes.Furthermore,this also accumulates scientific data for the subsequent comparative genomic analysis of cultivated Ganoderma spp.complex species in China.
作者
陈体强
徐晓兰
石林春
钟礼义
CHEN Ti-qiang;XU Xiao-lan;SHI Lin-chun;ZHONG Li-Yi(Institute of Edible&Medicinal Fungi Fujian Academy of Agricultural Sciences,Fuzhou 350014;Department of traditional Chinese medicine resources and bee products College of Animal Science College of Bee Science Fujian Agriculture and Forestry University,Fuzhou 350002;Institute of Medicinal Plant Development Chinese Academy of Medical Sciences,Beijing 100193;Fujian Wuping Mushroom Technical Advice Station,Longyan 364300)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第11期42-56,共15页
Biotechnology Bulletin
基金
福建省农业科学院创新团队建设项目(STIT2017-2-8,CXTD2021014-2)
福建省人民政府与中国农业科学院协同创新工程项目(XTCXGC2021003)。
关键词
紫芝
栽培品种
全基因组测序
基因组组分
基因功能注释
Zizhi(Ganoderma sinense complex)
cultivar
whole genome sequencing
genomic components
gene functional annotation
