摘要
利用生物信息学鉴定苦荞脂氧合酶(FtLOX)基因家族,分析其进化关系和组织表达模式,并进一步利用荧光定量PCR(qRT-PCR)技术分析FtLOX基因家族在铝胁迫应答中的表达模式.结果表明:苦荞FtLOX基因家族共包含10个成员(FtLOX1-FtLOX10),其可以分为9-LOX和13-LOX两个亚家族,且FtLOX的基因结构在同一个亚家族中是高度保守的.染色体定位和复制分析表明:FtLOX基因家族既有串联重复又有片段重复,而片段重复可能是苦荞FtLOXs进化的主要驱动力.qRT-PCR分析表明:6个FtLOX基因参与了苦荞铝胁迫的响应,其中FtLOX6、FtLOX8和FtLOX9可能在苦荞铝胁迫应答中起重要作用.这些结果为进一步探究FtLOXs在植物发育和胁迫应答中的功能奠定了良好的基础.
In the present study,we identified the FtLOX gene family in tartary buckwheat(Fagopyrum tataricum)and analyzed their evolutionary relationships and tissue expression patterns using bioinformatics method.In addition,the expression level of the FtLOX genes of tartary buckwheat under aluminum stress was investigated using quantitative RT-PCR(qRT-PCR).The results showed that ten putative FtLOX genes(FtLOX1–FtLOX10)were contained in tartary buckwheat,which can be divided into two subfamilies:9-LOX and 13-LOX The protein domains and gene structure compositions of FtLOXs appeared to be highly conserved among the subfamilies.Chromosomal location and duplication analysis showed that segmentally and tandemly duplicated gene pairs were observed among the FtLOXs and the segmental duplication may be a dominant driving force for FtLOX evolution in tartary buckwheat.The qRT-PCR analysis demonstrated that six of the FtLOXs are implicated in regulating the response of tartary buckwheat to aluminum,that FtLOX6,FtLOX8,and FtLOX9 play key roles of tartary buckwheat in responses to aluminum stress.This work lays a good foundation for further exploration of the potential functions of FtLOXs in plant development and in response to stresses in tartary buckwheat.
作者
仲雪婷
徐鸿
李鑫鑫
孙若嘉
付文英
李佳鹏
杨莲莲
吴酬飞
张立钦
王占旗
ZHONG Xueting;XU Hong;LI Xinxin;SUN Ruojia;FU Wenying;LI Jiapeng;YANG Lianlian;WU Choufei;ZHANG Liqin;WANG Zhanqi(School of Life Sciences,Huzhou University,Huzhou 313000,China;Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province,Huzhou University,Huzhou 313000,China)
出处
《湖州师范学院学报》
2021年第10期33-42,共10页
Journal of Huzhou University
基金
国家自然科学基金项目(31701759)
浙江省自然科学基金项目(LQ19C140001)
国家级大学生创新创业训练计划项目(202001165).
关键词
苦荞
脂氧合酶基因家族
系统发育
铝胁迫
基因表达
tartary buckwheat
lipoxygenase gene family
phylogenetics
aluminum stress
gene expression
