摘要
通过形态鉴定及ITS序列比对证实武夷山GL8栽培子实体为赤芝正品,在其ITS2区域中发现单碱基突变和插入位点。扫描电镜观察到原木栽培采收得到的GL8孢子粉纯度高,呈完整而饱满的椭圆形,而在破壁样品中可见其孢壁结构均已崩裂、破损。检测结果表明,破壁孢子粉中17种氨基酸总量可达13.04 g·100^(-1)·g^(-1),其中甲硫氨酸含量占比高达56.21%~57.64%;其粗脂肪含量高达32.1~32.4 g·100^(-1)·g^(-1),主要成分为以不饱和脂肪酸为主,包括油酸(C18∶1,61.4%)、亚油酸(C18∶2,13.3%)、棕榈酸(C16∶0,14.6%)和硬脂酸(C18∶0,4.2%)。通过临界CO_(2)流体萃取、采用气相色谱-质谱联用和高效液色谱分析,可以进一步确定孢子油中脂肪酸组分及是否存在齐墩果酸、熊果酸。
It was verified that the fruit bodies of Wuyishan Ganoderma lucidum cultivar GL8 was genuine G.lucidum by morphological taxonomy and ITS sequence comparison,while the SNP and single base insertion site were found in the ITS2 region.Under scanning electron microscope,it can be seen that the spores'powder obtained from log-cultivation was of high purity,completed and plump ellipse,while the sporoderm-broken spores showed those sporoderm structure had been cracked and damaged.The detection results showed the total amount of 17 kinds of amino acids in the wall-broken spores'powder was reached 13.04 g·100^(-1)·g^(-1),of which the content of methionine(Met)accounted for 56.21%-57.64%.While the contents of crude fat in the wall-broken spores were up to 32.1-32.4 g·100^(-1)·g^(-1),mainly composed of unsaturated fatty acids,including oleic acid(C18∶1,61.4%),linoleic acid(C18∶2,13.3%),palmitic acid(C16∶0,14.6%)and stearic acid(C18∶0,4.2%).The fatty acid components in the spore oil and the presence of oleanolic acid and ursolic acid were further determined by critical CO_(2) fluid extraction offline with GC-MS and HPLC analysis.
作者
毛景华
阮润晟
张泰顺
杨平
陈体强
MAO Jing-hua;RUAN Run-cheng;ZHANG Tai-shun;YANG Ping;CHEN Ti-qiang(Fujian Wuyishan Yuanshengtai Biomedical Co.,Ltd.,Nanping 354300,China;Institute of Traditional Medicine and Pharmacy,National Yang-Ming Chiao-Tung University,Taipei 000112,China;Institute of Edible&Medicinal Fungi,Fujian Academy of Agricultural Sciences,Fuzhou 350014,China)
出处
《中国食用菌》
2023年第4期67-74,共8页
Edible Fungi of China
基金
福建省科技厅公益类科研专项(2023R1106)。
关键词
赤芝
形态鉴定
ITS序列比对
孢子粉
破壁形态
化学成分
Ganoderma lucidum
morphological identification
ITS sequence alignment
spore powder
wall-broken micromorphology
chemical composition
