摘要
为研究白耙齿菌发酵茶的降血脂及保护肝脏功效,本实验采用高脂饲料诱导建立高血脂小鼠模型,将70只雄性昆明小鼠随机分为模型组、辛伐他汀组阳性对照组(10 mg/kg)、绿茶组(400 mg/kg),及白耙齿菌发酵茶高(400 mg/kg)、中(200 mg/kg)、低(100 mg/kg)剂量组,并另设空白对照组。空白对照组给予基础饲料,其余各组给予高脂饲料喂养。造模成功后,各组灌胃相应浓度的药物或茶汤。35 d后计算各组小鼠体重、肝重以及肝脏系数,检测血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白(LDL-C)胆固醇及谷丙转氨酶(AST)、谷草转氨酶(ALT)、碱性磷酸酶(ALP)含量,检测肝组织超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽(GSH)水平;并对肝脏组织进行病理学观察(HE)以及肝脏脂肪颗粒观察(油红O)。结果显示:白耙齿菌发酵茶高剂量组能显著降低高血脂小鼠TC、LDL-C、AST、ALT、ALP水平(P<0.05或P<0.01),其指标水平均接近于辛伐他汀组;极显著降低TG水平(P<0.01),并且与空白对照组TG水平相当;极显著升高HDL-C水平(P<0.01),其HDL-C水平接近辛伐他汀组;能显著降低高血脂小鼠肝脏中MDA含量并且低于辛伐他汀组,提高SOD活力接近空白对照组、GSH活力接近辛伐他汀组(P<0.05);能改善肝脏组织病理学结构,减少肝脏脂肪颗粒。上述实验结果表明:白耙齿菌发酵茶高剂量组能通过调节氧化应激等途径达到降血脂、改善脂肪肝和保护肝脏的作用。实验结果可为白耙齿菌发酵茶开发为抗氧化、降血脂、改善脂肪肝的功能菌茶提供一定的科学依据。
To study the effect of Irpex lacteus fermented tea on blood lipids and liver in mouse, 60 male KM mice were established hyperlipidemia model by high-fat diet induction, and then were randomly divided into six groups: Model group,simvastatin positive control group(10 mg/kg), green tea group(400 mg/kg) and high(400 mg/kg), medium(200 mg/kg), and low(100 mg/kg) dose Irpex lacteus fermented tea groups. And another 10 male KM mice were set up blank control group.The blank control group was given basic feeds, and the remaining groups were fed with high-fat feeds and gavage corresponding drugs. After 35 days, the body weight, liver weight and liver coefficient of each group were calculated.Serum total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP),superoxide dismutase(SOD), malondialdehyde(MDA) and glutathion peroxide(GSH) levels were detected, and morphological changes(HE) and liver fat particles(oil red O) in the liver of mice were observed. The results showed that high(400 mg/kg) dose Irpex lacteus fermented tea group could significantly reduce the levels of TC, LDL-C, AST, ALT,ALP and liver coefficient(P<0.05 or P<0.01), these indicators levels were close to the simvastatin group. High(400 mg/kg)dose Irpex lacteus fermented tea group extremely significantly reduced TG level(P<0.01), and with the blank control group TG level, and increased HDL-C levels(P<0.01) in hyperlipidemic mice, the level of HDL-C was close to simvastatin group.It also could significantly reduce MDA content in liver and hyperlipidemia mice below simvastatin group, and increase SOD and GSH activity, which were close to blank control group and simvastatin group, respectively(P<0.05), improve the degree of fatty liver histopathology, and reduce liver fat particles. These findings indicated that the high(400 mg/kg) dose Irpex lacteus fermented tea group had hypolipidemic, improved the
作者
韦炎冶
姜明国
邓丽姚
刘聪
高彩密
夏雨弘
刘洪存
陈林梦
杨立芳
WEI Yanye;JIANG Mingguo;DENG Liyao;LIU Cong;GAO Caimi;XIA Yuhong;LIU Hongcun;CHEN Linmeng;YANG Lifang(Guangxi Key Laboratory for Polysaccharide Materials and Modifications,School of Marine Sciences and Biotechnology,Guangxi University for Nationalities,Nanning 530008,China;Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center,Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,School of Chemistry and Chemical Engineering,Guangxi University for Nationalities,Nanning 530008,China)
出处
《食品工业科技》
CAS
北大核心
2021年第24期334-339,共6页
Science and Technology of Food Industry
基金
国家自然科学基金项目(81960164)
广西科技重大专项(桂科AA18242026)
广西民族大学研究生创新计划(gxun-chzb2018065)
广西科技基地和人才专项(桂科AD18126005)
广西高校中青年教师科研基础能力提升项目(2021KY0175)。
关键词
白耙齿菌
白耙齿菌茶
降血脂
脂肪肝
保护肝脏
Irpex lacteus
Irpex lacteus fermented tea
hypolipidemic
fatty liver
liver protection
