摘要
探讨苦菜黄酮(flavonoids from Sonchus oleraceus L.,FSOL)对高脂血症小鼠血脂代谢及肝脏的保护作用。选择60只雄性ICR小鼠随机分为6组:正常对照组、高脂模型组、阳性对照组(辛伐他汀,10 mg/kg)以及FSOL低剂量组(50 mg/kg)、中剂量组(100 mg/kg)、高剂量组(200 mg/kg),正常对照组饲喂基础饲料,其余组饲喂高脂饲料,连续饲喂5周后测定小鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)及肝脏中TC、TG、MDA、SOD等指标水平,并观察肝脏的组织形态变化。结果表明:FSOL能抑制高脂血症小鼠体质量的增长,降低血清中TC、TG、LDL-C、ALT、AST、MDA水平和动脉粥样硬化指数,并且降低肝脏中TC、TG、MDA水平和肝脏指数;另外,SOD和HDL-C水平都有不同程度提升,说明FSOL对高脂血症小鼠具有较好的降血脂和保肝作用,可能与其改善脂质代谢和增强机体抗氧化能力有关,这为FSOL的开发利用提供了一定的理论依据。
The aim of the present study was to observe the effect of flavonoids from Sonchus oleraceus L.(FSOL) on blood lipid metabolism and to further evaluate the hepatoprotective efficacy in hyperlipidemic mice. Sixty male ICR mice were randomly and equally divided into six groups: normal control group, high fat model group, positive control group(simvastatin, 10 mg/kg), and low-, middle-and high-dose FSOL(50, 100, and 200 mg/kg) groups. The mice in the normal control group were fed a basic diet, while those in the other groups were fed a high-fat diet. After 5 weeks of feeding, serum total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C), alanine aminotransferase(ALT), aspartate aminotransferase(AST), malondialdehyde(MDA), superoxide dismutase(SOD) and liver TC, TG, MDA and SOD were determined, and morphological changes in the liver of mice were observed. The results showed that FSOL could inhibit body mass gain in high-fat diet-fed mice, and reduce serum TC, TG, LDL-C, ALT, AST and MDA and atherosclerosis index(AI) levels as well as hepatic TC, TG and MDA and liver index. In addition, it could increase the levels of HDL-C and SOD to varying degrees. These findings indicated that FSOL has potent hypolipidemic and hepatoprotective effects in hyperlipidemic mice, and the underlying mechanism may be related to the improvement of liver lipid metabolism and the enhancement of antioxidant function. This study provides a theoretical basis for the development and utilization of FSOL.
作者
单科开
王鸿飞
许凤
罗洁
李艳霞
韩爱茹
邵兴锋
韦莹莹
SHAN Kekai;WANG Hongfei;XU Feng;LUO Jie;LI Yanxia;HAN Airu;SHAO Xingfeng;WEI Yingying(College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo 315211,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第19期231-236,共6页
Food Science
基金
浙江省自然科学基金项目(LY16C200003)
浙江省重点研发项目(2017C02021)
宁波市自然科学基金项目(2015A610273)
关键词
苦菜黄酮
高脂血症
血脂代谢
保肝作用
flavonoids from Sonchus oleraceus L.
hyperlipidemic
blood lipid metabolism
hepatoprotective efficacy
