摘要
目的分析白细胞介素-10(IL-10)基因多态性与肺结核易感性和抗结核治疗致肝损伤(ADILI)的相关性。方法选取2017年1月-2020年1月医院收治的肺结核患者120例(肺结核组)及同期健康体检者40名(对照组),采用序列特异性引物多聚酶链反应(PCR-SSP)技术对两组IL-10基因的3个单核苷酸多态性(SNP)位点[-819T/C(rs1800871)、-1082A/G(rs1800896)、-592A/C(rs1800872)]进行基因分型,并对比以上位点基因型、等位基因分布频率,将肺结核患者按是否发生ADILI分为ADILI患者15例、非ADILI患者105例,比较两亚组的临床资料及IL-10基因多态性。结果肺结核组-819T/C位点TT基因型频率、等位基因T频率高于对照组,肺结核组-1082A/G位点GG基因型频率及等位基因G频率高于对照组,肺结核组-592A/C位点基因型AA、AC、等位基因A频率高于对照组(P<0.05);-819T/C位点等位基因T、-1082A/G位点等位基因G、-592A/C位点等位基因A与肺结核易感性独立相关(P均<0.05);肺结核患者中,ADILI组的-819T/C位点TT基因型频率、等位基因T频率高于非ADILI组,ADILI组-1082A/G位点GG基因型频率及等位基因G频率高于非ADILI组,ADILI组-592A/C位点基因型AA、等位基因A频率高于非ADILI组(P<0.05);肺结核患者肝病史、饮酒史及-819T/C位点等位基因T、-592A/C位点等位基因A与ADILI的发生独立相关(P均<0.05)。结论IL-10基因-819T/C、-1082A/G、-592A/C位点多态性与肺结核易感性有关,其中-819T/C位点等位基因T、-592A/C位点等位基因A与ADILI的发生独立相关,可能是ADILI的易感基因。
OBJECTIVE To analyze the correlation of interleukin-10(IL-10)gene polymorphism with susceptibility to pulmonary tuberculosis(TB)and liver injury induced by anti-tuberculous therapy(ADILI).METHODS A total of 120patients with TB(TB group)admitted to Dongfang People's Hospital and the Second Affiliated Hospital of Hainan Medical College between Jan.2017and Jan.2020were selected as tuberculosis group,and 40healthy persons during the same period were selected as the control group.The sequence-specific primers polymerase chain reaction(PCR-SSP)technology was used to analyze three single nucleotide polymorphism(SNP)loci[-819T/C(rs1800871),-1802A/G(rs1800896),-592A/C(rs1800872)]of the two groups of IL-10genes for genotyping,and the genotypes and allele distribution frequencies of the above loci were compared.The pulmonary tuberculosis patients were divided into 15cases of ADILI group and 105cases of non-ADILI group according to whether ADILI occurred,and the clinical data and IL-10gene polymorphisms of the two subgroups were compared.RESULTS The genotype TT frequency and allele T frequency at-819T/C locus,the genotype GG frequency and allele G frequency at-1802A/G locus,as well as the genotypes AA,AC and allele A frequencies at-592A/C locus of TB group were all significantly higher than those of the control group(P<0.05).Allele T at-819T/C,allele G at-1802A/G and allele A at-592A/C were independently associated with susceptibility to TB(P<0.05).Among patients with TB,the genotype TT frequency and allele T frequency at-819T/C locus,genotype GG frequency and allele G frequency at-1802A/G locus,as well as the genotype AA frequency and allele A frequency at-592A/C locus of the ADILI group were significantly higher than those of the non-ADILI group(P<0.05).The history of liver disease,drinking history,allele T at-819T/C and allele A at-592A/C were independently associated with the occurrence of ADILI(P<0.05).CONCLUSIONThe polymorphisms of IL-10gene at-819T/C,-1802A/G and-592A/C loci were associated with increased susceptibili
作者
吉雪芳
吴坤亮
文小兰
赵仕玉
邵柳
JI Xue-fang;WU Kun-liang;WEN Xiao-lan;ZHAO Shi-yu;SHAO Liu(Dongfang People′s Hospital,Dongfang,Hainan 572600,China;不详)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2021年第19期2940-2944,共5页
Chinese Journal of Nosocomiology
基金
海南省东方市科学研究计划基金资助项目(DFKJXM2016003)。
关键词
白细胞介素-10
基因多态性
肺结核易感性
抗结核治疗致肝损伤
Interleukin-10
Gene polymorphism
Susceptibility to tuberculosis
Anti-tuberculous drug induced liver injury
