摘要
目的 研究白介素-10（IL-10）基因（-1082 G/A）多态性与肺结核病易感性的关系.方法 利用序列特异性引物多聚酶链反应（RCR-SSP）技术对40例肺结核患者及40例对照人群IL-10（-1082 G/A）多态性进行检测,并对其易感性进行分析.结果 对照组IL-10（-1082 G/A）AA基因型频率（0.825）显著高于患者组（0.55）（P＜0.01）.GA基因型患者组显著高于对照组（P＜0.05）.患者纽G等位基因频率（0.25）显著高于对照组（0.1）（P＜0.01）,RR为1.57,95%CI（1.10～2.24）,P＜0.05.结论 IL-10（-1082 G/A）多态性可能与肺结核病的易感性相关,因此其多态性检测可作为遗传标记进行检测,用于早期发现对肺结核病易感的高风险人群,对于结核病防治工作具有重要意义.
Objective To study the correlation between the polymorphism of interleukin 10 （ - 1082 G/A） gene and susceptibility to pulmonary tuberculosis （PTB）. Methods The polymorphism of interleukin 10 （ -1082 G/A） in 40 patients with PTB and 40 healthy control subjects was detected by using sequence - specific primers polymerase chain reaction （ PCR - SSP） and their susceptibility to development of PTB was analyzed. Results The genotypic frequency of AA of HL - 10 （ - 1082 G/A） in the control groups was significantly higher that of patient group （P 〈0. 01 ）. In contrast, the genotypic frequency of GA in the patient group significantly was higher than that in the control group（ P 〈 0.05 ）. A significant difference was found in G allele frequency of HL - 10 （ - 1082 G/A） between the two groups （0.25 vs O. 1, P 〈0.01）.The relative risk （RR） was 1.57 （95%CI 1.10 -2.24 ,P〈0.01）. Conclusion The polymorphism of interleukin 10 （ - 1082 G/A） gene may be associated with susceptibility to PTB. If can be used as a genetic marker for early identification of individuals at risk of PTB, and if important for the prevention of tuberculosis.
出处
《广东医学》
CAS
CSCD
北大核心
2007年第8期1243-1245,共3页
Guangdong Medical Journal
基金
广东省科技计划项目(编号:2004B36001045)
关键词
肺结核
IL-10基因
多态性
易感性
Pulmonary tuberculosis Interleukin 10 gene Polymorphism Susceptibility
