摘要
目的:探究丰富环境(EE)对血管性痴呆(VaD)大鼠海马区神经炎症和反应性星形胶质细胞的影响。方法:将36只成年雄性SD大鼠随机分为假手术组、模型组和模型+EE组各12只。用永久性双侧颈总动脉结扎术(2-VO)制备VaD模型。4周环境干预后,采用Morris水迷宫实验、原位末端转移酶标记(TUNEL)染色、实时定量聚合酶链反应(RT-qPCR)和免疫荧光双标法分别检测VaD大鼠的学习和记忆能力、海马神经元凋亡情况、炎症因子白细胞介素-1β(IL-1β)和反应性星形胶质细胞中神经胶质纤维酸性蛋白(GFAP)和脂质运载蛋白-2(LCN2)的表达变化。结果:模型+EE组在逃避潜伏期、目标象限所花时间和穿越平台次数上均表现得比模型组更好(P<0.05),但低于假手术组(P<0.05)。模型组大鼠神经元的TUNEL阳性细胞、IL-1β、GFAP和LCN2明显多于其余2组(P<0.05),除GFAP以外,假手术组TUNEL阳性细胞、IL-1β和LCN2的表达均显著低于模型+EE组(P<0.05)。结论:EE能减轻海马神经炎症和反应性星形胶质细胞的激活,同时伴随着VaD大鼠的认知功能的改善。
Objective:To investigate the effect of enriched environment(EE)on the hippocampus neuroinflammation and reactive astrocytes of vascular dementia(VaD)rats.Methods:Totally,36 adult male SD rats were randomly divided into sham operation group,model group and model+EE group with 12 rats in each group.2-vessel Occlusion(2-VO)surgery was used to make the rat model of VaD.After 4 weeks of environmental intervention,Morris water maze experiment,terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling(TUNEL)staining,real-time quantitative polymerase chain reaction(RT-qPCR),and double immunofluorescence labeling were used to detect the changes of learning and memory ability,the hippocampus neuron apoptosis,interleukin-1β(IL-1β)and glial fibers in acidic protein(GFAP)and lipocalin-2(LCN2)in reactive astrocytes of VaD rats.Results:The model+EE group performed better in escape latency,the time spent in target quadrant and the number of crossing platform than the model group(P<0.05),but still not as good as the sham group(P<0.05).The TUNEL positive cells,IL-1β,GFAP and LCN2 in the model group were significantly increased as compared with those in the rest two groups(P<0.05).The expression levels of TUNEL positive cells,IL-1βand LCN2 in the sham group were significantly lower than those in the model+EE group(P<0.05),except for GFAP.Conclusion:EE can reduce hippocampus neuroinflammation and the activation of reactive astrocytes,accompanied by improved cognitive function in VaD rats.
作者
林璐
周甜甜
郝赤子
廖维靖
Lin Lu;Zhou Tiantian;Hao Chizi(Department of Neurological Rehabilitation, Zhongnan Hospital of Wuhan University, Wuhan 430071, China)
出处
《中国康复》
2021年第11期643-648,共6页
Chinese Journal of Rehabilitation
基金
湖北省卫生计生委中医药科研项目(SZY2019003)。
